Beas-Zárate C, Schliebs R, Morales-Villagran A, Feria-Velasco A
Facultad de Ciencias, Universidad de Guadalajara, Jalisco, Mexico.
Epilepsy Res. 1989 Jul-Aug;4(1):20-7. doi: 10.1016/0920-1211(89)90054-5.
Adult rats (60 days old) were injected intraperitoneally with 5 mg/g monosodium L-glutamate (MSG). During the convulsive period (1 h after injection), uptake and release of [3H]norepinephrine (3H-NE) and [14C]dopamine (14C-DA) were measured in a crude synaptosomal fraction and in slices of cerebral cortex and caudate nucleus, respectively. A significant reduction of 3H-NE uptake was detected in cortical slices (by 42%) and in synaptosomal fraction (by 33%) of rats treated with MSG, whereas K+- stimulated 3H-NE release was decreased by 32% and 39% in brain slices and in a synaptosomal fraction of cerebral cortex, respectively, in comparison with animals injected with 0.9% NaCl aqueous solution (PSS). In the caudate nucleus, 14C-DA uptake was increased by 100% in brain slices and by 36% in the synaptosomal fraction following MSG administration, whereas K+- stimulated 14C-DA release was enhanced by 80% in slices and by 25% in synaptosomes as compared to PSS-injected rats. Data suggest that catecholaminergic neurotransmission may play an important role in the etiopathology of convulsions in the experimental model using MSG.
成年大鼠（60日龄）腹腔注射5毫克/克L-谷氨酸单钠（MSG）。在惊厥期（注射后1小时），分别在粗制突触体组分以及大脑皮层和尾状核切片中测量[3H]去甲肾上腺素（3H-NE）和[14C]多巴胺（14C-DA）的摄取和释放。与注射0.9%氯化钠水溶液（PSS）的动物相比，MSG处理的大鼠皮层切片（降低42%）和突触体组分（降低33%）中3H-NE摄取显著减少，而大脑切片和大脑皮层突触体组分中K+刺激的3H-NE释放分别降低32%和39%。在尾状核中，MSG给药后大脑切片中14C-DA摄取增加100%，突触体组分中增加36%，而与注射PSS的大鼠相比，切片中K+刺激的14C-DA释放增加80%，突触体中增加25%。数据表明，在使用MSG的实验模型中，儿茶酚胺能神经传递可能在惊厥的病因病理中起重要作用。
