Department of Chemistry, University of Virginia, Charlottesville, VA 22904.
Analyst. 2016 Sep 21;141(18):5256-5260. doi: 10.1039/c6an01469k. Epub 2016 Aug 18.
Glass insulated carbon-fiber microelectrodes (CFMEs) are standard tools for the measurement of neurotransmitters. However, electrodes are fabricated individually and the glass can shatter, limiting application in higher order mammals. Here, we developed a novel microelectrode batch fabrication method using a 3D-printed mold and polyimide resin insulating agent. The 3D-printed mold is low cost, customizable to change the electrode shape, and allows 40 electrodes to be made simultaneously. The polyimide resin is biocompatible, quick to cure, and does not adhere to the plastic mold. The electrodes were tested for the response to dopamine with fast-scan cyclic voltammetry both in vitro and in vivo and performed similarly to traditional glass-insulated electrodes, but with lower background currents. Thus, polyimide-insulated electrodes can be mass-produced using a 3D-printed mold and are an attractive alternative for making cheap, biocompatible microelectrodes.
玻璃绝缘碳纤维微电极（CFMEs）是测量神经递质的标准工具。然而，电极是逐个制造的，而且玻璃可能会破裂，限制了其在较高等哺乳动物中的应用。在这里，我们使用 3D 打印模具和聚酰亚胺树脂绝缘剂开发了一种新的微电极批量制造方法。3D 打印模具成本低，可定制以改变电极形状，并且可以同时制造 40 个电极。聚酰亚胺树脂具有生物相容性，固化速度快，并且不与塑料模具粘连。使用快速扫描循环伏安法对体外和体内的多巴胺反应对这些电极进行了测试，它们的性能与传统的玻璃绝缘电极相似，但背景电流较低。因此，使用 3D 打印模具可以批量生产聚酰亚胺绝缘电极，这是制造廉价、生物相容的微电极的一种有吸引力的替代方案。
