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基于单壁碳纳米角的荧光能量共振转移适体传感器平台用于黄曲霉毒素B1的检测

Single-Walled Carbon Nanohorn-Based Fluorescence Energy Resonance Transfer Aptasensor Platform for the Detection of Aflatoxin B1.

作者信息

Fan Yiting, Yang Huanhuan, Li Jiaxin, Amin Khalid, Lyu Bo, Jing Wendan, Wang Sainan, Fu Hongling, Yu Hansong, Guo Zhijun

机构信息

College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China.

Division of Soybean Processing, Soybean Research & Development Center, Chinese Agricultural Research System, Changchun 130118, China.

出版信息

Foods. 2023 Jul 28;12(15):2880. doi: 10.3390/foods12152880.

DOI:10.3390/foods12152880
PMID:37569149
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10417297/
Abstract

Aflatoxin B1 (AFB1) is one of the most contaminated fungal toxins worldwide and is prone to cause serious economic losses, food insecurity, and health hazards to humans. The rapid, on-site, and economical method for AFB1 detection is need of the day. In this study, an AFB1 aptamer (AFB1-Apt) sensing platform was established for the detection of AFB1. Fluorescent moiety (FAM)-modified aptamers were used for fluorescence response and quenching, based on the adsorption quenching function of single-walled carbon nanohorns (SWCNHs). Basically, in our constructed sensing platform, the AFB1 specifically binds to AFB1-Apt, making a stable complex. This complex with fluorophore resists to be adsorbed by SWCNHs, thus prevent SWCNHs from quenching of fluorscence, resulting in a fluorescence response. This designed sensing strategy was highly selective with a good linear response in the range of 10-100 ng/mL and a low detection limit of 4.1 ng/mL. The practicality of this sensing strategy was verified by using successful spiking experiments on real samples of soybean oil and comparison with the enzyme-linked immunosorbent assay (ELISA) method.

摘要

黄曲霉毒素B1（AFB1）是全球污染最严重的真菌毒素之一，容易给人类造成严重的经济损失、粮食不安全和健康危害。当下需要一种快速、现场且经济的AFB1检测方法。在本研究中，建立了一种用于检测AFB1的AFB1适配体（AFB1-Apt）传感平台。基于单壁碳纳米角（SWCNHs）的吸附猝灭功能，使用荧光部分（FAM）修饰的适配体进行荧光响应和猝灭。基本上，在我们构建的传感平台中，AFB1特异性结合AFB1-Apt，形成稳定的复合物。这种带有荧光团的复合物抵抗被SWCNHs吸附，从而防止SWCNHs猝灭荧光，导致荧光响应。这种设计的传感策略具有高度选择性，在10 - 100 ng/mL范围内具有良好的线性响应，检测限低至4.1 ng/mL。通过对大豆油实际样品进行成功的加标实验并与酶联免疫吸附测定（ELISA）方法进行比较，验证了这种传感策略的实用性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/595631b527af/foods-12-02880-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/4634a6156b24/foods-12-02880-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/02914aed63bd/foods-12-02880-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/5047b9572a47/foods-12-02880-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/f6e438ba15d2/foods-12-02880-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/8b0a43aa0e60/foods-12-02880-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/6f1c2e8b892a/foods-12-02880-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/595631b527af/foods-12-02880-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/4634a6156b24/foods-12-02880-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/02914aed63bd/foods-12-02880-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/5047b9572a47/foods-12-02880-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/f6e438ba15d2/foods-12-02880-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/8b0a43aa0e60/foods-12-02880-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/6f1c2e8b892a/foods-12-02880-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd9/10417297/595631b527af/foods-12-02880-g006.jpg

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