Stock C E, Bates R, Lindsay K S, Edmonds D K, Fraser L R
Chelsea Hospital for Women, London, UK.
J Reprod Fertil. 1989 May;86(1):401-11. doi: 10.1530/jrf.0.0860401.
The effect of human follicular fluid (FF) on the incidence of spontaneous acrosome reactions (AR) in human spermatozoa was examined over a 24-25 h period using electron microscopy. Suspensions of motile spermatozoa were prepared by a swim-up method in Earle's medium, known to support in-vitro fertilization. After adjusting the concentration to 10 x 10(6) cells/ml, suspensions were diluted 1:1 with medium (control) or FF, the latter giving a final concentration of 50% FF. In addition, at 5 h and 24 h an aliquant of the control suspension was removed, diluted 1:1 with FF and incubated for 1 h; the three suspensions were examined at 6 h and 25 h. Continuous exposure to 50% FF stimulated the AR, the effect being significant (P less than 0.001) at 25 h. However, the 1-h short exposure of spermatozoa to FF did not produce an increase in AR, even after 24 h preincubation. In a separate series of experiments, the effect of continuous incubation for 24 h in increasing concentrations of FF was investigated. A significant linear dose-dependent effect on the AR was observed with all concentrations assessed (P less than 0.01 for 12.5% FF and P less than 0.001 for 25, 50, 75 and 100% FF, compared with FF-free control). Therefore, human FF can stimulate the AR, but only after a continuous exposure to FF. A short exposure to FF, even after 24 h preincubation, does not trigger an increased AR response.
采用电子显微镜技术，在24 - 25小时内检测了人卵泡液（FF）对人精子自发顶体反应（AR）发生率的影响。通过在已知支持体外受精的Earle's培养基中上浮法制备活动精子悬液。将浓度调整至10×10⁶个细胞/ml后，悬液用培养基（对照）或FF 1:1稀释，后者最终浓度为50% FF。此外，在5小时和24小时时，从对照悬液中取出一份，用FF 1:1稀释并孵育1小时；在6小时和25小时时检查这三种悬液。持续暴露于50% FF可刺激AR，在25小时时这种作用显著（P < 0.001）。然而，精子对FF 1小时的短时间暴露即使在预孵育24小时后也不会使AR增加。在另一系列实验中，研究了在不断增加浓度的FF中持续孵育24小时的影响。在所评估的所有浓度下均观察到对AR有显著的线性剂量依赖性作用（与无FF对照相比，12.5% FF时P < 0.01，25%、50%、75%和100% FF时P < 0.001）。因此，人FF可刺激AR，但只有在持续暴露于FF后才行。即使预孵育24小时后，对FF的短时间暴露也不会引发AR反应增加。
