Pilikian S, Mimouni P
Laboratoire de Biologie de la Reproduction et du Développement, Faculté de Médecine, Lyon, France.
Int J Androl. 1988 Dec;11(6):465-72. doi: 10.1111/j.1365-2605.1988.tb01020.x.
The in-vitro kinetics of the acrosome reaction and the survival of human spermatozoa were studied under different capacitating conditions. Human preovulatory follicular fluid (FF), isotonic BWW (N-BWW) and hypertonic BWW (H-BWW) were tested. Motile sperm selected by migration in these media were examined after 1, 3, 5 and 22 h of incubation under 5% CO2. The kinetics of the reaction in the population of live, morphologically normal sperm was dependent on both the culture medium and time of incubation. In the first hour, the mean percentage of acrosome-reacted sperm in H-BWW and FF was significantly greater than in N-BWW. The proportion of reacted cells increased significantly after 3 h in N-BWW (P = 0.001), after 5 h in FF (P = 0.03) and after 22 h in H-BWW (P = 0.01). A significant decrease in sperm viability was registered at 3 and 22 h of incubation (P less than 0.002) in all media. These results demonstrate that both H-BWW and FF stimulate the acrosome reaction while survival is optimal in the latter.
在不同的获能条件下，研究了顶体反应的体外动力学和人类精子的存活情况。测试了人排卵前卵泡液（FF）、等渗BWW（N - BWW）和高渗BWW（H - BWW）。在5%二氧化碳条件下孵育1、3、5和22小时后，检查通过在这些培养基中迁移筛选出的活动精子。活的、形态正常的精子群体中反应的动力学取决于培养基和孵育时间。在第一个小时，H - BWW和FF中顶体反应精子的平均百分比显著高于N - BWW。在N - BWW中3小时后（P = 0.001）、FF中5小时后（P = 0.03）和H - BWW中22小时后（P = 0.01），反应细胞的比例显著增加。在所有培养基中，孵育3小时和22小时时精子活力显著下降（P小于0.002）。这些结果表明，H - BWW和FF都能刺激顶体反应，而在卵泡液中精子存活最佳。
