Coetzee K, Olmedo J, Lombard C J
Department of Obstetrics and Gynecology, University of Stellenbosch, Tygerberg, South Africa.
J Assist Reprod Genet. 1994 Oct;11(9):470-3. doi: 10.1007/BF02215710.
This study was conducted to determine the fertility predictive value of acrosome reaction rates and indices (induced minus control) of human spermatozoa. By comparison these outcomes with in vitro fertilization success. The effect of oocyte-cumulus complex exposure on the induction of the acrosome reaction was also analyzed. Patients attending our assisted reproduction unit for infertility treatment were included in the study. Acrosome reactions were determined on ethanol-permeabilized smears using FITC-conjugated Pisum sativum agglutinin. The acrosome reaction inducing agent used was calcium ionophore A23187 (10 microM/ml).
Poor correlations were found between all the acrosome reaction rates and indices and in vitro fertilization. The presence of oocyte-cumulus complexes had no effect on the spontaneous acrosome reactions, but had a significant effect on the inducibility of the acrosome reaction. Exposure to oocyte-cumulus complexes resulted in the mean percentage sperm induced to be 7.8% (SE = 3.1%) higher compared to the control samples.
Acrosome reaction rates and indices were therefore found to have no significant value in the prediction of male fertility and/or in vitro fertilization success. This study did, however, show that exposure to oocyte-cumulus complexes significantly increases the inducible sperm population.
本研究旨在确定人类精子顶体反应率和指标（诱导值减去对照值）对生育能力的预测价值。通过将这些结果与体外受精成功率进行比较，还分析了卵母细胞-卵丘复合体暴露对顶体反应诱导的影响。纳入在我们辅助生殖单元接受不孕症治疗的患者进行研究。使用异硫氰酸荧光素（FITC）偶联的豌豆凝集素，在乙醇通透涂片上测定顶体反应。所用的顶体反应诱导剂为钙离子载体A23187（10微摩尔/毫升）。
所有顶体反应率和指标与体外受精之间的相关性均较差。卵母细胞-卵丘复合体的存在对自发顶体反应没有影响，但对顶体反应的诱导性有显著影响。与对照样本相比，暴露于卵母细胞-卵丘复合体导致诱导精子的平均百分比高7.8%（标准误=3.1%）。
因此发现顶体反应率和指标在预测男性生育能力和/或体外受精成功率方面没有显著价值。然而，本研究表明，暴露于卵母细胞-卵丘复合体可显著增加可诱导的精子数量。
