Suard I M, Collins V P, Ignacio V, Jacque C M
Neurochimie, Inserm U 134, Hôpital de la Salpêtrière, Paris, France.
J Neurosci Res. 1989 Jun;23(2):172-9. doi: 10.1002/jnr.490230207.
Brain fragments containing embryonic rabbit glia were implanted into the brains of newborn mice. The hosts developed an astroglial reaction around the transplants and along the needle tracks. Transplant-derived astrocytes were identified in the operated brain by their expression of rabbit GFAP. During the first few days post-implantation (PI) glial cells were exchanged between the transplant and the host. Less than 3 to 5 days PI, the transplant was extensively invaded by host astrocytes. Xenogeneic astroglial cells were first detected 10 days PI in the immediate proximity of the transplant. At 2 to 11 weeks, they could be detected either close to or at distance from the point of implantation. Most often, transplant-derived astrocytes presented a morphology similar to that of neighboring host astrocytes. Xenogeneic glial cells were found to participate in various types of astroglial features: sub-pial, pericapillary, fibrous, and protoplasmic. This morphological integration suggests that they are physiologically integrated, at least to a certain degree, in the host tissue. In spite of their integration into the host, xenogeneic astrocytes disappear after 3 months without signs of an inflammatory reaction.
将含有胚胎兔神经胶质细胞的脑片段植入新生小鼠的大脑中。宿主在移植周围以及针道周围出现了星形胶质细胞反应。通过兔胶质纤维酸性蛋白（GFAP）的表达，在手术的大脑中鉴定出了移植来源的星形胶质细胞。在植入后的最初几天，移植组织与宿主之间发生了胶质细胞的交换。植入后不到3至5天，移植组织就被宿主星形胶质细胞广泛侵入。在植入后10天，首次在移植组织紧邻处检测到异种星形胶质细胞。在2至11周时，在距植入点近处或远处都能检测到它们。大多数情况下，移植来源的星形胶质细胞呈现出与相邻宿主星形胶质细胞相似的形态。发现异种胶质细胞参与了各种类型的星形胶质细胞特征：软膜下、毛细血管周围、纤维状和原浆性。这种形态学整合表明它们至少在一定程度上在生理上整合到了宿主组织中。尽管异种星形胶质细胞整合到了宿主中，但3个月后它们消失了，没有炎症反应的迹象。
