Yang X Y, Hosseini J M, Ruddel M E, Elin R J
Clinical Pathology Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Md.
Magnesium. 1989;8(2):100-5.
The population of mononuclear blood cells (MBCs) separated by a discontinuous gradient from healthy normal volunteers consists of lymphocytes (L), monocytes (M) and relatively few granulocytes (G). We attempted to remove the phagocytic cells (M and G) by pretreatment of the blood with carbonyl iron particles (CIP). In 50 volunteers (21 males and 29 females, ages 17-75, mean 33.3 years), we determined the content and concentration of Mg in MBCs before and after pretreatment with CIP. The results (mean +/- SEM) show a significant decrease in the MBC Mg content (from 3.12 +/- 0.08 to 2.39 +/- 0.05 fmol/cell; p less than 0.0001), concentration (from 10.4 +/- 0.2 to 9.6 +/- 0.3 mmol/l; p less than 0.004) and M percentage (from 14.5 +/- 1.0 to 1.0 +/- 0.2%; p less than 0.0001) after CIP pretreatment. The L percentage significantly increased from 81.6 +/- 1.0 to 96.1 +/- 0.2% (p less than 0.0001) after CIP pretreatment. These date suggest that M have both a larger content and concentration of Mg than L.
通过不连续梯度从健康正常志愿者中分离出的单核血细胞（MBCs）群体由淋巴细胞（L）、单核细胞（M）和相对较少的粒细胞（G）组成。我们试图通过用羰基铁颗粒（CIP）对血液进行预处理来去除吞噬细胞（M和G）。在50名志愿者（21名男性和29名女性，年龄17 - 75岁，平均33.3岁）中，我们测定了用CIP预处理前后MBCs中镁的含量和浓度。结果（平均值±标准误）显示，CIP预处理后，MBCs中镁的含量（从3.12±0.08降至2.39±0.05 fmol/细胞；p＜0.0001）、浓度（从10.4±0.2降至9.6±0.3 mmol/L；p＜0.004）和M百分比（从14.5±1.0降至1.0±0.2%；p＜0.0001）均显著降低。CIP预处理后，L百分比从81.6±1.0显著增加至96.1±0.2%（p＜0.0001）。这些数据表明，M的镁含量和浓度均高于L。
