人淋巴细胞密度纯化过程中缺乏亚类选择。
Lack of subclass selection during density purification of human lymphocytes.
作者信息
Häyry P, Tötterman T H, Ranki A
出版信息
Clin Exp Immunol. 1977 May;28(2):341-6.
Abstract
The percentage of acid alpha-naphthyl acetate esterase (ANAE) marker-carrying lymphocytes (T-lymphocytes) was determined directly from the blood, after density purification of lymphocytes over Ficoll-Isopaque and removal of monocytes by iron powder plus magnet. The effect of Ficoll-Isopaque centrifugation was insignificant; neither did the iron powder plus magnet procedure increase the relative proportion of the T cells in these preparations. We therefore conclude that in optimal conditions there is no subclass-specific enrichment of T or B lymphocytes during the purification procedure.
摘要
在通过Ficoll-泛影葡胺对淋巴细胞进行密度纯化并用铁粉加磁铁去除单核细胞后，直接从血液中测定携带酸性α-萘乙酸酯酶（ANAE）标记的淋巴细胞（T淋巴细胞）的百分比。Ficoll-泛影葡胺离心的效果不显著；铁粉加磁铁的操作也没有增加这些制剂中T细胞的相对比例。因此，我们得出结论，在最佳条件下，纯化过程中不存在T或B淋巴细胞的亚类特异性富集。
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Ann N Y Acad Sci. 1971 Dec 31;190:420-31. doi: 10.1111/j.1749-6632.1971.tb13552.x.
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Evidence for individual human peripheral blood lymphocytes bearing both B and T cell markers.个体人类外周血淋巴细胞同时携带B和T细胞标志物的证据。
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Receptors for complement and immunoglobulin on human leukemic cells and human lymphoblastoid cell lines.人类白血病细胞和人类淋巴母细胞系上补体和免疫球蛋白的受体
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Cell surface markers for human T and B lymphocytes.人类T淋巴细胞和B淋巴细胞的细胞表面标志物。
Eur J Immunol. 1974 Apr;4(4):302-10. doi: 10.1002/eji.1830040414.
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Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells.人类T和B淋巴细胞的表面标志物。I. 大量与绵羊红细胞形成非免疫性玫瑰花结的淋巴细胞。
J Exp Med. 1972 Aug 1;136(2):207-15. doi: 10.1084/jem.136.2.207.
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Interaction of aggregated -globulin with B lymphocytes.聚集的γ球蛋白与B淋巴细胞的相互作用。
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