Chagovets Vtaliy, Kononikhin Aleksey, Starodubtseva Nataliia, Kostyukevich Yury, Popov Igor, Frankevich Vladimir, Nikolaev Eugene
Department of System Biology in Reproduction, Federal State Budget Institution "Research Center for Obstetrics, Gynecology and Perinatology", 4 Oparin Street, Moscow 117997, Russian Federation.
Department of System Biology in Reproduction, Federal State Budget Institution "Research Center for Obstetrics, Gynecology and Perinatology", 4 Oparin Street, Moscow 117997, Russian Federation. >Moscow Institute of Physics and Technology, 141700 Dolgoprudnyi, Moscow Region, Russian Federation.
Eur J Mass Spectrom (Chichester). 2016;22(3):123-6. doi: 10.1255/ejms.1425.
The importance of high-resolution mass spectrometry for the correct data interpretation of a direct tissue analysis is demonstrated with an example of its clinical application for an endometriosis study. Multivariate analysis of the data discovers lipid species differentially expressed in different tissues under investigation. High-resolution mass spectrometry allows unambiguous separation of peaks with close masses that correspond to proton and sodium adducts of phosphatidylcholines and to phosphatidylcholines differing in double bond number.
通过高分辨率质谱在子宫内膜异位症研究临床应用的实例，证明了其对直接组织分析正确数据解读的重要性。对数据进行多变量分析发现了在所研究的不同组织中差异表达的脂质种类。高分辨率质谱能够明确分离质量相近的峰，这些峰对应于磷脂酰胆碱的质子和钠加合物以及双键数量不同的磷脂酰胆碱。
