通过串联和傅里叶变换离子回旋共振质谱法对具有质子化和钠化分子干扰质量的磷脂酰胆碱进行相对定量。

Relative quantitation of phosphatidylcholines with interfered masses of protonated and sodiated molecules by tandem and Fourier-transform ion cyclotron resonance mass spectrometry.

作者信息

Chagovets Vitaliy, Kononikhin Aleksey, Tokoreva Alisa, Bormotov Denis, Starodubtseva Nataliia, Kostyukevich Yury, Popov Igor, Frankevich Vladimir, Nikolaev Eugene

机构信息

1 V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.

2 V. L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.

出版信息

Eur J Mass Spectrom (Chichester). 2019 Apr;25(2):259-264. doi: 10.1177/1469066718799992.

DOI:10.1177/1469066718799992

PMID:31018698

Abstract

The present work deals with deconvolution of interfered peaks of protonated and sodiated phosphatidylcholines to solve the problem of relative quantitation of these compounds. The method was developed based on the fact that adducts of phosphatidylcholines with proton and sodium ion give unique characteristic peaks in tandem mass spectrometry. Ultra-high-resolution Fourier-transform ion cyclotron resonance mass spectrometry was used to resolve interfered peaks and thus validate the results of tandem mass spectrometry-based deconvolution. Applicability of the method was tested with synthesized phosphatidylcholines and applied for tissue-spray analysis of the clinical samples and demonstrated good correlation (0.996) between MS/MS and Fourier-transform ion cyclotron resonance mass spectrometry results.

摘要

本研究致力于对质子化和钠化磷脂酰胆碱的干扰峰进行去卷积，以解决这些化合物的相对定量问题。该方法基于磷脂酰胆碱与质子和钠离子的加合物在串联质谱中产生独特特征峰这一事实而开发。使用超高分辨率傅里叶变换离子回旋共振质谱来解析干扰峰，从而验证基于串联质谱去卷积的结果。该方法的适用性通过合成的磷脂酰胆碱进行测试，并应用于临床样本的组织喷雾分析，结果表明串联质谱和傅里叶变换离子回旋共振质谱的结果之间具有良好的相关性（0.996）。