粪便代谢组学在囊性纤维化中能发挥作用吗？
Is there a role for stool metabolomics in cystic fibrosis?
作者信息
Kaakoush Nadeem O, Pickford Russell, Jaffe Adam, Ooi Chee Y
机构信息
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia.
Bioanalytical Mass Spectrometry Facility, University of New South Wales, Sydney, New South Wales, Australia.
出版信息
Pediatr Int. 2016 Aug;58(8):808-11. doi: 10.1111/ped.13063.
A number of studies utilizing metabolomics have focused on the pathophysiology of cystic fibrosis (CF) lung disease. Here, we performed fecal metabolomics on pancreatic insufficient (PI) and sufficient (PS) children with CF and compared them with healthy controls (HC). Fecal metabolomics can differentiate between PS-CF and PI-CF. We identified a potential biomarker of disease severity or cystic fibrosis transmembrane conductance regulator function (m/z, 463.247; retention time, 0.570717 min) that discriminates between HC versus PS-CF versus PI-CF. We also identified lipoyl-GMP as a potential novel inflammatory biomarker, and elevation in fecal glycerol 1,2-didodecanoate 3-tetradecanoate may provide clues to the pathogenesis of intestinal inflammation. For the first time, we demonstrate the potential applications of fecal metabolomics in CF.
一些利用代谢组学的研究聚焦于囊性纤维化（CF）肺部疾病的病理生理学。在此，我们对患有CF的胰腺功能不全（PI）和胰腺功能正常（PS）儿童进行了粪便代谢组学分析，并将其与健康对照（HC）进行比较。粪便代谢组学能够区分PS-CF和PI-CF。我们鉴定出一种疾病严重程度或囊性纤维化跨膜传导调节因子功能的潜在生物标志物（质荷比，463.247；保留时间，0.570717分钟），它能够区分HC与PS-CF以及PI-CF。我们还将硫辛酸-GMP鉴定为一种潜在的新型炎症生物标志物，粪便中甘油1,2-十二烷酸酯3-十四烷酸酯的升高可能为肠道炎症的发病机制提供线索。我们首次证明了粪便代谢组学在CF中的潜在应用。
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