Giri Lalit, Jugran Arun Kumar, Bahukhandi Amit, Dhyani Praveen, Bhatt Indra D, Rawal Ranbeer Singh, Nandi Shyamal Kumar, Dhar Uppeandra
G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, Uttarakhand, 263 643, India.
University School of Environment Management, GGS Indraprastha University, Sector-16C, Dwarka, New Delhi, 110078, India.
Appl Biochem Biotechnol. 2017 Jan;181(1):267-282. doi: 10.1007/s12010-016-2211-8. Epub 2016 Aug 23.
Habenaria edgeworthii Hook. f. ex Collett is an important terrestrial orchid used in different Ayurvedic formulations. In the present study, variations among morphological, phytochemical and molecular markers were assessed. A significant difference was observed among populations using morphological traits. Inter-simple sequence repeat (ISSR) data revealed lower genetic diversity at population level (He = 0.207) as compared to species level (He = 0.334). Analysis of molecular variance (AMOVA) indicates 74 % variation among populations and 26 % within population. Tuber extracts showed significantly (p < 0.05) higher total phenolics and flavonoids among the populations. Antioxidant activity determined by 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant power (FRAP) assays exhibited considerable antioxidant potential. Furthermore, the associations between molecular and morphological and phytochemical attributes were studied using multiple regression analysis (MRA). Several ISSR fragments were associated with some morphological and phytochemical traits. These ISSR fragments can be useful for breeding programme of the species when no other genetic information, such as linkage maps and quantitative trait loci, is available.
埃氏玉凤花（Habenaria edgeworthii Hook. f. ex Collett）是一种重要的地生兰花，用于多种阿育吠陀配方中。在本研究中，评估了形态、植物化学和分子标记之间的变异。利用形态特征观察到不同种群之间存在显著差异。简单重复序列区间（ISSR）数据显示，与物种水平（He = 0.334）相比，种群水平的遗传多样性较低（He = 0.207）。分子方差分析（AMOVA）表明，74%的变异存在于种群之间，26%的变异存在于种群内部。块茎提取物在不同种群中总酚和黄酮类物质含量显著更高（p < 0.05）。通过2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除、1,1-二苯基-2-苦基肼（DPPH）自由基清除和铁还原抗氧化能力（FRAP）测定的抗氧化活性显示出相当大的抗氧化潜力。此外，使用多元回归分析（MRA）研究了分子与形态和植物化学属性之间的关联。几个ISSR片段与一些形态和植物化学性状相关。当没有其他遗传信息，如连锁图谱和数量性状位点时，这些ISSR片段可用于该物种的育种计划。
