Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto 606-8522, Japan.
Biotechnology Research Department, Kyoto Prefectural Agriculture, Forestry and Fisheries Technology Center, Seika, Kyoto 619-0244, Japan.
Int J Mol Sci. 2020 Dec 30;22(1):311. doi: 10.3390/ijms22010311.
Floral organs have evolved from leaves for reproduction, and the morphological analyses help to understand the plant diversity and evolution. (syn. ) is a terrestrial orchid living in wetlands in Japan, Russia, South Korea, and China. The habitats of this plant in Japan have been reduced because of environmental destruction and overexploitation, and thus it is on the Red List of Japan as a Near Threatened species. One of the three petals of the flower is called a lip or labellum, which resembles a flying white bird, egret, or white heron, with its proposed function being to attract pollinators. To understand the diversity of plants in different areas, we examined the lip morphology and phylogeny of populations from eight habitats in the Kinki area, Japan. The complex shapes of the lips were quantified and presented as a radar chart, enabling characterization of the morphological difference among populations. Phylogenetic analysis with microsatellite markers that we generated showed the variation of genetic diversity among populations, suggesting the different degrees of inbreeding, outbreeding, and vegetative propagation. Our approach offers a basic method to characterize the morphological and genetic diversity in natural populations.
花器官从叶子进化而来，用于繁殖，形态分析有助于了解植物多样性和进化。 （syn. ）是一种陆生兰花，生活在日本、俄罗斯、韩国和中国的湿地中。由于环境破坏和过度开发，这种植物在日本的栖息地已经减少，因此被列为日本近危物种红色名录。花的三个花瓣之一称为唇瓣或唇瓣，它类似于一只飞翔的白色鸟、白鹭或白鹳，其功能是吸引传粉者。为了了解不同地区 植物的多样性，我们研究了来自日本关西地区八个栖息地的种群的唇形态和系统发育。将复杂的唇形进行量化，并以雷达图的形式呈现，从而能够描述种群之间的形态差异。我们生成的微卫星标记的系统发育分析显示了种群之间遗传多样性的变化，表明了不同程度的近亲繁殖、远缘繁殖和营养繁殖。我们的方法为描述自然种群的形态和遗传多样性提供了一种基本方法。
