羊水中环（组氨酸-脯氨酸）样免疫反应性的鉴定与表征。

Identification and characterization of cyclo(His-Pro)-like immunoreactivity in amniotic fluid.

作者信息

Hilton C W, Wolf G C, Wilber J F, Prasad C, Rogers D

机构信息

Department of Medicine, LSU Medical Center, New Orleans 70112.

出版信息

Peptides. 1989 Mar-Apr;10(2):299-301. doi: 10.1016/0196-9781(89)90033-8.

DOI:10.1016/0196-9781(89)90033-8

PMID:2755872

Abstract

Amniotic fluid (AF) from 25 term pregnancies was analyzed for cyclo(His-Pro)-like immunoreactivity (CHP-LI). CHP-LI was detected in all AF samples and was indistinguishable from synthetic CHP by immunoidentity, by gel chromatography on Sephadex G-25, and by high pressure liquid chromatography. The mean concentration of CHP-LI in AF was 13,622 +/- 1288 pg/ml (+/- SE) and concentrations were not altered by maternal labor. Plasma concentrations of CHP-LI were similar in 4 pregnant and 4 control subjects [2260 +/- 432 pg/ml vs. 2162 +/- 419 pg/ml (+/- SE), respectively]. We conclude that 1) CHP-LI is readily detected in AF from term pregnancies and is indistinguishable from synthetic CHP, and 2) concentrations of CHP-LI in human AF are significantly higher than concentrations of maternal plasma CHP-LI, suggesting CHP AF originates by mechanisms other than diffusion from maternal plasma.

摘要

对来自25例足月妊娠的羊水（AF）进行了环（组氨酸-脯氨酸）样免疫反应性（CHP-LI）分析。在所有羊水样本中均检测到CHP-LI，通过免疫鉴定、在Sephadex G-25上的凝胶色谱法以及高压液相色谱法，其与合成CHP无法区分。羊水中CHP-LI的平均浓度为13,622±1288 pg/ml（±标准误），且浓度不受产妇分娩的影响。4名孕妇和4名对照受试者的血浆CHP-LI浓度相似[分别为2260±432 pg/ml和2162±419 pg/ml（±标准误）]。我们得出结论：1）在足月妊娠的羊水中很容易检测到CHP-LI，且其与合成CHP无法区分；2）人羊水中CHP-LI的浓度显著高于母体血浆CHP-LI的浓度，这表明羊水CHP的产生机制并非源于母体血浆的扩散。