嗜热史密斯芽孢杆菌模式菌株DSM 4216(T)的全基因组序列
Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216(T).
作者信息
Bosma Elleke F, Koehorst Jasper J, van Hijum Sacha A F T, Renckens Bernadet, Vriesendorp Bastienne, van de Weijer Antonius H P, Schaap Peter J, de Vos Willem M, van der Oost John, van Kranenburg Richard
机构信息
Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands ; Present address: The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Kgs. Lyngby, 2800 Denmark.
Laboratory of Systems and Synthetic Biology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
出版信息
Stand Genomic Sci. 2016 Aug 24;11(1):52. doi: 10.1186/s40793-016-0172-8. eCollection 2016.
Bacillus smithii is a facultatively anaerobic, thermophilic bacterium able to use a variety of sugars that can be derived from lignocellulosic feedstocks. Being genetically accessible, it is a potential new host for biotechnological production of green chemicals from renewable resources. We determined the complete genomic sequence of the B. smithii type strain DSM 4216(T), which consists of a 3,368,778 bp chromosome (GenBank accession number CP012024.1) and a 12,514 bp plasmid (GenBank accession number CP012025.1), together encoding 3880 genes. Genome annotation via RAST was complemented by a protein domain analysis. Some unique features of B. smithii central metabolism in comparison to related organisms included the lack of a standard acetate production pathway with no apparent pyruvate formate lyase, phosphotransacetylase, and acetate kinase genes, while acetate was the second fermentation product.
史密斯芽孢杆菌是一种兼性厌氧嗜热细菌，能够利用多种可从木质纤维素原料中获得的糖类。由于其基因易于操作，它是利用可再生资源生物技术生产绿色化学品的潜在新宿主。我们测定了史密斯芽孢杆菌模式菌株DSM 4216(T)的完整基因组序列，该序列由一条3368778 bp的染色体（GenBank登录号CP012024.1）和一个12514 bp的质粒（GenBank登录号CP012025.1）组成，共编码3880个基因。通过RAST进行的基因组注释得到了蛋白质结构域分析的补充。与相关生物体相比，史密斯芽孢杆菌中心代谢的一些独特特征包括缺乏标准的乙酸盐产生途径，没有明显的丙酮酸甲酸裂解酶、磷酸转乙酰酶和乙酸激酶基因，而乙酸盐是第二种发酵产物。
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