Anderson G W, Saluzzo J F, Ksiazek T G, Smith J F, Ennis W, Thureen D, Peters C J, Digoutte J P
USAMRIID, Ft. Detrick, Frederick, MD 21701-5011.
Res Virol. 1989 Mar-Apr;140(2):129-38. doi: 10.1016/s0923-2516(89)80090-1.
Several cell cultures and animals were compared for their relative sensitivity as primary isolation systems for Rift Valley fever virus (RVFV) and to determine if virulence characteristics of the isolates were altered in these systems. Eleven human sera from known cases of Rift Valley fever (RVF) were obtained from the 1987 epidemic in Mauritania and served as the source of virus for these studies. Sera were inoculated directly into cell cultures (Vero, C6/36 and DBS-FRhL-2) and animals (ICR suckling mice, Lak:LVG(SYR) hamsters and WF rats) concurrently. The cell lines provided a quick method to propagate, quantitate and identify these specimens without prior adaption. The isolates were highly virulent for suckling mice and hamsters, but not for WF rats, even after cell culture passage, which indicated that the Mauritanian isolates more closely resembled those strains from sub-Saharan Africa than those from the 1977-78 Egyptian epidemic.
对几种细胞培养物和动物作为裂谷热病毒（RVFV）的初次分离系统的相对敏感性进行了比较，并确定在这些系统中分离株的毒力特征是否发生改变。从1987年毛里塔尼亚疫情中获取了11份已知裂谷热（RVF）病例的人类血清，作为这些研究的病毒来源。将血清同时直接接种到细胞培养物（Vero、C6/36和DBS-FRhL-2）和动物（ICR乳鼠、Lak:LVG(SYR)仓鼠和WF大鼠）中。这些细胞系提供了一种无需预先适应即可繁殖、定量和鉴定这些标本的快速方法。分离株对乳鼠和仓鼠具有高毒力，但对WF大鼠没有，即使经过细胞培养传代也是如此，这表明毛里塔尼亚分离株与来自撒哈拉以南非洲的毒株比与1977 - 1978年埃及疫情中的毒株更相似。
