从人类外周血单个核细胞中分离出裂谷热病毒：毛里塔尼亚疫情
Isolation of Rift Valley fever virus from human peripheral blood mononuclear cells: Mauritanian epidemic.
作者信息
Sarthou J L, Jouan A, Le Guenno B, Philippe B, Riou O, Lena P, Knauert F K, Peters C J, Digoutte J P
机构信息
Cellular Immunology, Institut Pasteur de Dakar.
出版信息
Res Virol. 1989 May-Jun;140(3):263-70. doi: 10.1016/s0923-2516(89)80103-7.
During the Mauritanian Rift Valley fever (RVF) epidemic of 1987, peripheral blood mononuclear cells (PBMC) were studied from 78 sick patients. RVF virus (RVFV) was isolated in 5 cases, on Aedes pseudoscutellaris AP61, from both PBMC and serum. Among the 78 cases studied, RVF was proven in 19 cases (24.3%) by specific IgM detection, and in 12 cases (15.3%) by virus isolation from serum, of which 3 also exhibited anti-RVF IgM. Among the 5 PBMC-positive RVFV cases, 2 strains were isolated in the presence of specific IgM from patients presenting with neurologic signs. These observations raised the question as to the role of specific IgM in cellular infection, and suggest that, in certain cases, mononuclear cells may promote RVFV dissemination into brain cells. Further investigations need to be undertaken to determine the RVFV receptor expressed on PBMC membranes.
在1987年毛里塔尼亚裂谷热（RVF）疫情期间，对78名患病患者的外周血单个核细胞（PBMC）进行了研究。在5例患者中，从PBMC和血清中分离出裂谷热病毒（RVFV），分离毒株为伪盾蚊AP61。在所研究的78例病例中，通过特异性IgM检测确诊19例（24.3%）为裂谷热，通过血清病毒分离确诊12例（15.3%）为裂谷热，其中3例也检测出抗RVF IgM。在5例PBMC阳性的RVFV病例中，从出现神经系统症状的患者中，在特异性IgM存在的情况下分离出2株病毒。这些观察结果引发了关于特异性IgM在细胞感染中的作用的问题，并表明在某些情况下，单核细胞可能促进RVFV向脑细胞的传播。需要进一步开展研究以确定PBMC膜上表达的RVFV受体。
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