Lai K N, Lai F M, Tam J S
Department of Medicine, Chinese University, Hong Kong.
Am J Clin Pathol. 1989 Aug;92(2):159-65. doi: 10.1093/ajcp/92.2.159.
The nature of hepatitis B virus (HBV) antigens in HBV-associated glomerulonephritides was investigated in 7 hepatitis B surface antigen (HBsAg) carriers with membranous nephropathy, 16 HBsAg carriers with mesangial IgA nephropathy, and 1 HBsAg carrier with a mixed picture of membranous and IgA nephropathies. Consecutive frozen sections of renal biopsy specimens were stained with polyclonal and monoclonal antibodies against HBV antigens. Glomerular capillary deposits of HBeAg and HBcAg were detected in 66% and 57% of renal biopsies from HBsAg carriers with membranous nephropathy by monoclonal and polyclonal antibodies, respectively. The discrepancy in the immunofluorescence findings resulted from the cross-reactivity of the polyclonal anti-HBcAg antiserum because it contains both anti-HBcAg and anti-HBeAg activities. Mesangial deposits of HBsAg were detected in 40% and 21% of renal biopsies from HBsAg carriers with mesangial IgA nephropathy by polyclonal and monoclonal antibodies, respectively. The authors' study confirms that HBeAg is the predominant HBV antigen deposited in HBV-associated membranous nephropathy, and glomerular HBsAg deposits are detected in some HBsAg carrier with mesangial IgA nephropathy. Careful testing and evaluation of each antibody are necessary to prevent misinterpretation.
对7例患膜性肾病的乙肝表面抗原（HBsAg）携带者、16例患系膜IgA肾病的HBsAg携带者以及1例患膜性和IgA肾病混合型的HBsAg携带者，研究了乙肝病毒（HBV）相关肾小球肾炎中HBV抗原的性质。用抗HBV抗原的多克隆抗体和单克隆抗体对肾活检标本的连续冰冻切片进行染色。在患膜性肾病的HBsAg携带者的肾活检标本中，分别用单克隆抗体和多克隆抗体检测到，66%的标本有HBeAg肾小球毛细血管沉积，57%的标本有HBcAg肾小球毛细血管沉积。免疫荧光结果的差异是由于多克隆抗HBcAg抗血清的交叉反应性所致，因为它同时含有抗HBcAg和抗HBeAg活性。在患系膜IgA肾病的HBsAg携带者的肾活检标本中，分别用多克隆抗体和单克隆抗体检测到，40%的标本有系膜HBsAg沉积，21%的标本有系膜HBsAg沉积。作者的研究证实，HBeAg是沉积在HBV相关膜性肾病中的主要HBV抗原，并且在一些患系膜IgA肾病的HBsAg携带者中检测到肾小球HBsAg沉积。为防止误解，对每种抗体进行仔细检测和评估是必要的。
