2010 - 2015年卢旺达南部恶性疟原虫与青蒿素抗性相关的K13多态性
Artemisinin Resistance-Associated K13 Polymorphisms of Plasmodium falciparum in Southern Rwanda, 2010-2015.
作者信息
Tacoli Costanza, Gai Prabhanjan P, Bayingana Claude, Sifft Kevin, Geus Dominik, Ndoli Jules, Sendegeya Augustin, Gahutu Jean Bosco, Mockenhaupt Frank P
机构信息
Institute of Tropical Medicine and International Health, Charité-Universitätsmedzin Berlin, Berlin, Germany.
University Teaching Hospital of Butare, Butare, Rwanda.
出版信息
Am J Trop Med Hyg. 2016 Nov 2;95(5):1090-1093. doi: 10.4269/ajtmh.16-0483. Epub 2016 Aug 29.
Emerging artemisinin resistance is a threat to global malaria control. Mutations in the Plasmodium falciparum Kelch 13 (K13) propeller domain confer artemisinin resistance and constitute molecular markers for its detection and monitoring. We sequenced 222 P. falciparum isolates obtained from community children in the Huye District of southern Rwanda in 2010, 2014, and 2015 to investigate the presence of K13 polymorphisms. No polymorphisms were observed in 2010 but they were present in 2.5% and 4.5% in 2014 and 2015, respectively. In 2015, two isolates showed candidate K13 resistance mutations (P574L and A675V), which are common in southeast Asia and associated with delayed parasite clearance. K13 polymorphisms in southern Rwanda are infrequent but include variants associated with artemisinin resistance. Establishing correlations with local treatment response and in vitro resistance assays are needed in addition to further monitoring K13 polymorphisms in the study area.
青蒿素耐药性的出现对全球疟疾控制构成威胁。恶性疟原虫 Kelch 13（K13）螺旋桨结构域的突变赋予青蒿素耐药性，并构成其检测和监测的分子标志物。我们对 2010 年、2014 年和 2015 年从卢旺达南部胡耶区社区儿童中获得的 222 株恶性疟原虫分离株进行测序，以调查 K13 多态性的存在情况。2010 年未观察到多态性，但 2014 年和 2015 年分别有 2.5%和 4.5%的多态性。2015 年，两株分离株显示出候选的 K13 耐药性突变（P574L 和 A675V），这些突变在东南亚很常见，并与寄生虫清除延迟有关。卢旺达南部的 K13 多态性并不常见，但包括与青蒿素耐药性相关的变体。除了在研究区域进一步监测 K13 多态性外，还需要建立与当地治疗反应和体外耐药性检测的相关性。
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