Costas M, Owen R J
J Med Microbiol. 1987 Jun;23(4):303-11. doi: 10.1099/00222615-23-4-303.
A total of 31 cultures representing 22 different strains of Streptobacillus moniliformis has been characterised by unidimensional SDS-PAGE of cell proteins. The isolates were from sporadic and outbreak cases of human infection in the USA, UK and France, as well as mouse, turkey and rat isolates from various countries. The protein patterns, which contained 40-50 discrete bands, were highly reproducible and were used as the basis for a numerical analysis. Three clusters were obtained at the 86% similarity (S) level and these were further divided to give a total of seven clusters at the 90% S level. S. moniliformis strains, irrespective of the original host or geographical location of isolation, had a characteristic protein profile that enabled them to be easily distinguished from allied unclassifiable organisms. The analysis showed that Haverhill fever strains can be clearly distinguished from rat-bite fever strains. Protein-band differences amongst the latter strains corresponded with different geographical locations. We conclude that high resolution PAGE combined with computerised analysis of protein profiles provides the basis for typing clinical isolates of S. moniliformis. Seven PAGE types were identified and reference strains for each have been designated as a basis for future studies.
通过对细胞蛋白进行一维SDS-PAGE，对代表22种不同念珠状链杆菌菌株的31种培养物进行了特征分析。这些分离株来自美国、英国和法国人类感染的散发病例和暴发疫情，以及来自不同国家的小鼠、火鸡和大鼠分离株。蛋白质图谱包含40 - 50条离散条带，具有高度可重复性，并用作数值分析的基础。在86%的相似性（S）水平上获得了三个聚类，在90%的S水平上进一步划分，总共得到七个聚类。念珠状链杆菌菌株，无论其原始宿主或分离的地理位置如何，都具有特征性的蛋白质谱，这使得它们能够很容易地与相关的无法分类的生物体区分开来。分析表明，哈弗希尔热菌株可以与鼠咬热菌株清楚地区分开来。后一种菌株之间的蛋白带差异与不同的地理位置相对应。我们得出结论，高分辨率PAGE结合蛋白质谱的计算机分析为念珠状链杆菌临床分离株的分型提供了基础。确定了七种PAGE类型，并为每种类型指定了参考菌株，作为未来研究的基础。
