Sakagami H, Ikeda M, Konno K
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Biochem Biophys Res Commun. 1989 Jul 31;162(2):597-603. doi: 10.1016/0006-291x(89)92352-8.
The mouse macrophage-like cell line, J774.1, spontaneously released differentiation-inducing factor(s). When these cells were treated with a protein-bound polysaccharide, PSK, significantly higher amounts of differentiation-inducing activity were accumulated in the culture supernatant. PSK directly stimulated human myelogenous leukemic cell differentiation induced by J774.1 conditioned medium or by tumor necrosis factor. Among four subfractions of PSK, only the highest molecular weight fraction (MW greater than 200 kD) exerted such a stimulating effect.
小鼠巨噬细胞样细胞系J774.1能自发释放分化诱导因子。当用一种蛋白结合多糖PSK处理这些细胞时，培养上清液中积累的分化诱导活性显著增加。PSK直接刺激由J774.1条件培养基或肿瘤坏死因子诱导的人骨髓性白血病细胞分化。在PSK的四个亚组分中，只有分子量最高的组分（分子量大于200 kD）具有这种刺激作用。
