Sakagami H, Kim F, Konno K
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Anticancer Res. 1990 May-Jun;10(3):697-702.
A protein-bound polysaccharide, PSK, extracted from the mycelium of Coriolus versicolor (Fr.) Quel, stimulated the iodination (incorporation of radioactive iodine into an acid-insoluble fraction) of human peripheral blood polymorphonuclear cells (PMN), human promyelocytic leukemic HL-60 cells and human myeloblastic leukemic ML-1 cells. In contrast, PSK did not significantly increase the iodination of other cultured cell lines (U-937, THP-1, L-929, T98G, BALB 3T3). The PSK stimulation of iodination of both PMN and HL-60 cells depended on incubation time and temperature, and was significantly suppressed by the presence of myeloperoxidase inhibitors. Among various PSK subfractions, the highest molecular weight fraction (MW greater than 200 kD), or the fraction precipitated at pH 4.0-4.5, stimulated the iodination most. In contrast, natural and chemically modified glucans had little or no stimulation activity. The active PSK subfractions synergistically enhanced TNF stimulation of PMN iodination. The data suggest the presence of some unique components in PSK which directly stimulate the iodination of myeloperoxidase-positive cells.
从云芝（Coriolus versicolor (Fr.) Quel）菌丝体中提取的一种蛋白结合多糖——PSK，可刺激人外周血多形核白细胞（PMN）、人早幼粒细胞白血病HL - 60细胞和人髓母细胞白血病ML - 1细胞的碘化作用（将放射性碘掺入酸不溶性部分）。相比之下，PSK对其他培养细胞系（U - 937、THP - 1、L - 929、T98G、BALB 3T3）的碘化作用没有显著增加。PSK对PMN和HL - 60细胞碘化作用的刺激取决于孵育时间和温度，并且会被髓过氧化物酶抑制剂的存在显著抑制。在各种PSK亚组分中，分子量最高的组分（分子量大于200 kD）或在pH 4.0 - 4.5沉淀的组分对碘化作用的刺激最强。相比之下，天然和化学修饰的葡聚糖几乎没有刺激活性。活性PSK亚组分协同增强了TNF对PMN碘化作用的刺激。数据表明PSK中存在一些独特的成分，可直接刺激髓过氧化物酶阳性细胞的碘化作用。
