Peric Kacarevic Zeljka, Snajder Darija, Maric Andela, Bijelic Nikola, Cvijanovic Olga, Domitrovic Robert, Radic Radivoje
Department of Anatomy and Neuroscience, Faculty of Medicine University of Osijek, J. Huttlera 4, 3100 Osijek, Croatia.
Department of Anatomy and Neuroscience, Faculty of Medicine University of Osijek, J. Huttlera 4, 3100 Osijek, Croatia; Clinical Institute of Nuclear Medicine and Radiation Protection, University Hospital Centre Osijek, J. Huttlera 4, 31000, Osijek, Croatia.
Acta Histochem. 2016 Sep;118(7):711-721. doi: 10.1016/j.acthis.2016.08.002. Epub 2016 Aug 28.
In obesity, bone marrow adiposity increases and proinflammatory cytokines excretion activates RANK/RANKL/OPG system, which leads to increased bone resorption. The aim of this study was to analyze trabecular and cortical bone parameters in animals exposed to the high-fat diet in utero and after lactation. Skeletal organ of interest was the fifth lumbar vertebra, which is not exposed to biomechanical loading in rats. Further aims were to determine TNF-α and IL-6 serum concentrations, and the intensity of the TNF-α immunohistochemical staining in the bone marrow. Ten female Sprague Dawley rats, nine weeks old, were randomly divided in two groups and fed either standard laboratory chow or food rich in saturated fatty acids during five weeks, and then mated with genetically similar male subjects. After birth and lactation male offsprings from both groups were divided in four subgroups depending on the diet they were fed until twenty-two weeks of age. The highest cholesterol and triglyceride concentration were found in both groups of offsprings fed with high-fat diet. The lowest trabecular bone volume, lowest trabecular number and highest trabecular separation were found in offsprings fed with high-fat diet of mothers on standard laboratory chow. The same group of offsprings was also characterized by the highest intensity of TNF-α immunostaining in the bone marrow and the highest TNF-α serum concentration, which suggest that this proinflammatory cytokine has interfered with bone metabolism, possibly by stimulation of bone resorption, which led to inadequate trabecular bone development and bone modeling of the fifth lumbar vertebra.
在肥胖状态下，骨髓脂肪增多，促炎细胞因子的分泌激活了RANK/RANKL/OPG系统，导致骨吸收增加。本研究的目的是分析在子宫内和哺乳期暴露于高脂饮食的动物的小梁骨和皮质骨参数。感兴趣的骨骼器官是第五腰椎，其在大鼠中未受到生物力学负荷影响。进一步的目的是测定血清TNF-α和IL-6浓度，以及骨髓中TNF-α免疫组化染色的强度。将10只9周龄的雌性Sprague Dawley大鼠随机分为两组，在五周内分别喂食标准实验室饲料或富含饱和脂肪酸的食物，然后与基因相似的雄性大鼠交配。出生和哺乳后，两组的雄性后代根据其喂养的饮食分为四个亚组，直至22周龄。在两组喂食高脂饮食的后代中均发现了最高的胆固醇和甘油三酯浓度。在喂食标准实验室饲料的母亲所生且喂食高脂饮食的后代中，发现了最低的小梁骨体积、最低的小梁数量和最高的小梁间距。同一组后代的骨髓中TNF-α免疫染色强度最高，血清TNF-α浓度也最高，这表明这种促炎细胞因子可能通过刺激骨吸收干扰了骨代谢，导致第五腰椎小梁骨发育不足和骨塑形异常。
