Šnajder Darija, Perić Kačarević Željka, Grgić Anđela, Bijelić Nikola, Fenrich Matija, Belovari Tatjana, Radić Radivoje
a Department of Anatomy and Neuroscience , Josip Juraj Strossmayer University of Osijek , Osijek , Croatia.
b Clinical Institute of Nuclear Medicine and Radiation Protection, University Hospital Osijek , Osijek , Croatia.
J Matern Fetal Neonatal Med. 2019 Jun;32(11):1838-1846. doi: 10.1080/14767058.2017.1419181. Epub 2018 Jan 2.
Adipose tissue expansion can occur through several different ways and, under certain conditions, can be connected with chronic inflammation. TNF-α is one of the important cytokines involved in this process. Prolonged inflammation in obesity can lead to obesity-related insulin resistance and tissue dysfunction. The aim of our study was to investigate how different combination of maternal and postnatal diet affects offspring adipose tissue morphology and adipose tissue TNF-α expression.
Ten female Sprague Dawley rats, 9 weeks old, were randomly divided into two groups and fed either standard laboratory chow or food rich in saturated fatty acids during 6 weeks and then mated with the same male rat. After birth and lactation male rat offspring from both groups were divided into four subgroups depending on the diet they were fed until 22 weeks old. Samples of white adipose tissue were taken from the subcutaneous, epididymal, and perirenal fat pad. On tissue sections, histomorphometric analysis was conducted using CellProfiler program v 2.1.1, and immunohistochemical staining for TNF-α was performed.
Greater mean surface area of subcutaneous and epididymal adipocytes was found in groups of male rat offspring with altered diet. In perirenal adipose tissue, the highest number of adipocytes was measured in the group where both mother and offspring were fed a high-fat diet. Adipocyte staining intensity for TNF-α did not differ significantly between the groups.
Together with our previously published data, our results lead to the conclusion that alteration of postnatal diet can lead to TNF-α and adipocyte morphology changes.
脂肪组织扩张可通过几种不同方式发生，在某些情况下，还可能与慢性炎症相关。肿瘤坏死因子-α（TNF-α）是参与这一过程的重要细胞因子之一。肥胖状态下的长期炎症可导致肥胖相关的胰岛素抵抗和组织功能障碍。本研究的目的是探讨母代和产后饮食的不同组合如何影响子代脂肪组织形态及脂肪组织TNF-α表达。
将10只9周龄的雌性斯普拉格-道利大鼠随机分为两组，在6周内分别给予标准实验室饲料或富含饱和脂肪酸的食物，然后与同一只雄性大鼠交配。两组的雄性大鼠子代在出生及哺乳期后，根据其至22周龄时所喂食的饮食分为四个亚组。从皮下、附睾及肾周脂肪垫采集白色脂肪组织样本。在组织切片上，使用CellProfiler 2.1.1版程序进行组织形态计量分析，并进行TNF-α的免疫组化染色。
饮食改变的雄性大鼠子代组中，皮下和附睾脂肪细胞的平均表面积更大。在肾周脂肪组织中，母代和子代均喂食高脂饮食的组中测得的脂肪细胞数量最多。各亚组间TNF-α的脂肪细胞染色强度无显著差异。
结合我们之前发表的数据，我们的结果得出结论，产后饮食的改变可导致TNF-α和脂肪细胞形态变化。
