Fitzpatrick F A, Bundy G L
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2689-93. doi: 10.1073/pnas.75.6.2689.
Antibodies that recognized the prostaglandin (PG) E structure were elicited from rabbits. 9-Deoxy-9-methylene-PGF2alpha, a stable isosteric mimic of PGE2, was conjugated to two different protein immunogens and the immune response system was duped into producing antibodies with poor recognition for prostaglandins other than the hapten mimic (9-deoxy-9-methylene-PGF2alpha) and its isosteric counterparts (PGE1 and PGE2). With this procedure, crossreaction that would ordinarily arise from the chemical or metabolic instability of an authentic PGE2 immunogen was avoided. Antibodies raised against a keyhole limpet hemocyanin conjugate of 9-deoxy-9-methylene-PGF2alpha had an average intrinsic association constant, Ko = 2.6 X 10(9) liters.mole-1, for PGE2. Crossreaction was low for a number of related prostaglandins, and a sensitive radioimmunoassay procedure with a detection limit of 6 pg was developed.
能识别前列腺素（PG）E结构的抗体是从兔子体内诱导产生的。9-脱氧-9-亚甲基-PGF2α是一种稳定的PGE2等电子类似物，它与两种不同的蛋白质免疫原偶联，免疫反应系统被诱导产生对除半抗原类似物（9-脱氧-9-亚甲基-PGF2α）及其等电子类似物（PGE1和PGE2）之外的前列腺素识别能力较差的抗体。通过这种方法，避免了由真实PGE2免疫原的化学或代谢不稳定性通常会引起的交叉反应。针对9-脱氧-9-亚甲基-PGF2α的钥孔血蓝蛋白偶联物产生的抗体对PGE2的平均内在缔合常数Ko = 2.6×10⁹升·摩尔⁻¹。对许多相关前列腺素的交叉反应较低，并开发了一种检测限为6皮克的灵敏放射免疫测定方法。
