Hugill Alison, Shimomura Kenju, Cox Roger D
Mammalian Genetics Unit, Medical Research Council Harwell, Harwell Science and Innovation Campus, Oxfordshire, United Kingdom.
Department of Medical Electrophysiology, Fukushima Medical University, School of Medicine, Fukushima, Japan.
Curr Protoc Mouse Biol. 2016 Sep 1;6(3):256-271. doi: 10.1002/cpmo.14.
This article describes detailed protocols for in vitro measurements of insulin function and secretion in isolated mouse islets for the analysis of glucose homeostasis. We specify a method of enzyme digestion and hand picking to isolate and release the greatest number of high quality islets from the pancreas of the mouse. We describe an effective method for generating dynamic measurements of insulin secretion using a perifusion assay including a detailed protocol for constructing a peristaltic pump and tubing assembly. In addition we describe an alternative and simple technique for measuring insulin secretion using static incubation of isolated islets. © 2016 by John Wiley & Sons, Inc.
本文描述了用于体外测量分离的小鼠胰岛中胰岛素功能和分泌以分析葡萄糖稳态的详细方案。我们指定了一种酶消化和手工挑选的方法，以从小鼠胰腺中分离并释放出数量最多的高质量胰岛。我们描述了一种使用灌流测定法动态测量胰岛素分泌的有效方法，包括构建蠕动泵和管路组件的详细方案。此外，我们还描述了一种使用分离胰岛的静态孵育来测量胰岛素分泌的替代且简单的技术。© 2016约翰威立父子公司。
