Rahnama H, Sattarzadeh A, Kazemi F, Ahmadi N, Sanjarian F, Zand Z
Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran; Gil Nanogene Biotech Co., Research and Development Department, Tehran, Iran.
Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Anal Biochem. 2016 Nov 15;513:68-76. doi: 10.1016/j.ab.2016.08.029. Epub 2016 Aug 31.
Recent updates on Magnetic Nano-Particles (MNPs) based separation of nucleic acids have received more attention due to their easy manipulation, simplicity, ease of automation and cost-effectiveness. It has been indicated that DNA molecules absorb on solid surfaces via hydrogen-bonding, and hydrophobic and electrostatic interactions. These properties highly depend on the surface condition of the solid support. Therefore, surface modification of MNPs may enhance their functionality and specification. In the present study, we functionalized Fe3O4 nano-particle surface utilizing SiO2 and TiO2 layer as Fe3O4/SiO2 and Fe3O4/SiO2/TiO2 and then compare their functionality in the adsorption of plasmid DNA molecules with the naked Fe3O4 nano-particles. The result obtained showed that the purity and amount of DNA extracted by Fe3O4 coated by SiO2 or SiO2/TiO2 were higher than the naked Fe3O4 nano-particles. Furthermore, we obtained pH 8 and 1.5 M NaCl as an optimal condition for desorption of DNA from MNPs. The result further showed that, 0.2 mg nano-particle and 10 min at 55 °C are the optimal conditions for DNA desorption from nano-particles. In conclusion, we recommended Fe3O4/SiO2/TiO2 as a new MNP for separation of DNA molecules from biological sources.
基于磁性纳米颗粒（MNPs）的核酸分离技术的最新进展因其操作简便、简单易行、易于自动化且成本效益高而受到更多关注。研究表明，DNA分子通过氢键、疏水作用和静电相互作用吸附在固体表面。这些性质高度依赖于固体载体的表面状况。因此，MNPs的表面修饰可能会增强其功能和特异性。在本研究中，我们利用SiO2和TiO2层对Fe3O4纳米颗粒表面进行功能化，形成Fe3O4/SiO2和Fe3O4/SiO2/TiO2，然后将它们与裸露的Fe3O4纳米颗粒在吸附质粒DNA分子方面的功能进行比较。结果表明，用SiO2或SiO2/TiO2包覆的Fe3O4提取的DNA的纯度和量高于裸露的Fe3O4纳米颗粒。此外，我们获得了pH 8和1.5 M NaCl作为从MNPs上解吸DNA的最佳条件。结果还表明，0.2 mg纳米颗粒和55°C下10分钟是从纳米颗粒上解吸DNA的最佳条件。总之，我们推荐Fe3O4/SiO2/TiO2作为一种从生物来源分离DNA分子的新型MNP。
