Rahnama H, Sattarzadeh A, Kazemi F, Ahmadi N, Sanjarian F, Zand Z
Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran; Gil Nanogene Biotech Co., Research and Development Department, Tehran, Iran.
Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Data Brief. 2016 Oct 26;9:807-811. doi: 10.1016/j.dib.2016.10.013. eCollection 2016 Dec.
In this dataset we integrated figures related to bacterial transformation using pBI121 plasmid and complementary analysis for magnetic nanoparticles (MNPs) characterizations. The structural map of pBI121 plasmid was drawn by Vector NTI software using the complete sequence of binary vector pBI121. bacteria transformed using pBI121 plasmid and were grown on the selection media containing kanamycin. MNPs were characterized by energy dispersive spectroscopy (EDS) and transmission electron microscopy (TEM). Finally, the overall efficiency of different MNPs (FeO, FeO/SiO, FeO/SiO/TiO) in plasmid DNA isolation was compared using gel electrophoresis analysis. The data supplied in this article supports the accompanying publication "Comparative study of three magnetic nano-particles (FeSO, FeSO/SiO, FeSO/SiO/TiO) in plasmid DNA extraction" (H. Rahnama, A. Sattarzadeh, F. Kazemi, N. Ahmadi, F. Sanjarian, Z. Zand, 2016) [1].
在这个数据集中，我们整合了与使用pBI121质粒进行细菌转化相关的图表，以及用于磁性纳米颗粒（MNP）表征的补充分析。pBI121质粒的结构图是通过Vector NTI软件利用二元载体pBI121的完整序列绘制的。使用pBI121质粒转化细菌，并在含有卡那霉素的选择培养基上培养。通过能量色散光谱（EDS）和透射电子显微镜（TEM）对磁性纳米颗粒进行表征。最后，使用凝胶电泳分析比较了不同磁性纳米颗粒（FeO、FeO/SiO、FeO/SiO/TiO）在质粒DNA分离中的整体效率。本文提供的数据支持了随附的出版物《三种磁性纳米颗粒（FeSO、FeSO/SiO、FeSO/SiO/TiO）在质粒DNA提取中的比较研究》（H. Rahnama、A. Sattarzadeh、F. Kazemi、N. Ahmadi、F. Sanjarian、Z. Zand，2016年）[1]。
