DPI Water, NSW Department of Primary Industries (DPI), Elizabeth Macarthur Agricultural Institute, Private Bag 4008, Narellan, New South Wales, 2567, Australia; Centre for Ecosystem Science, University of New South Wales, Sydney, New South Wales, 2052, Australia.
UNSW Water Research Centre, School of Civil and Environmental Engineering, University of New South Wales, Sydney, New South Wales, 2052, Australia; The bioMASS Lab, School of Chemical Engineering, University of New South Wales, Sydney, New South Wales, 2052, Australia.
Water Res. 2016 Nov 15;105:22-33. doi: 10.1016/j.watres.2016.08.051. Epub 2016 Aug 26.
A YSI EXO2 water quality sonde fitted with fluorometric sensors for chlorophyll-a (Chl-a) and phycocyanin (CPC) was used to determine its applicability in cyanobacterial quantification in three small urban ponds in Sydney, Australia displaying considerable variations in cyanobacterial community composition and abundance, as well as eukaryotic algae, turbidity and chromophoric dissolved organic matter. CPC and Chl-a measured in situ with the instrument was compared against laboratory measures of cyanobacterial biovolume over two summer sampling periods. A good correlation was found between CPC and total cyanobacterial biovolume in two of the three ponds. The poor correlation in the third was due to the frequent dominance of picoplanktonic sized cyanobacteria. CPC did not correlate well with cell counts, and Chl-a was a poor measure of cyanobacterial presence. The relationship between CPC measured by fluorometry varied according to the dominant cyanobacterial taxa present in the ponds at any one time. Fluorometry has good potential for use in environmental monitoring of cyanobacterial biovolume, but may need to be based on predetermined relations applicable to local water bodies. Management guidelines based on CPC concentrations would also enhance the usefulness of in situ CPC measurements.
使用配备荧光传感器的 YSI EXO2 水质探头来测定叶绿素-a（Chl-a）和藻蓝蛋白（CPC），以确定其在澳大利亚悉尼的三个小型城市池塘中对蓝藻定量的适用性。这些池塘的蓝藻群落组成和丰度以及真核藻类、浊度和色源溶解有机物质存在显著差异。仪器原位测量的 CPC 和 Chl-a 与两个夏季采样期间实验室测量的蓝藻生物量进行了比较。在其中两个池塘中，CPC 与总蓝藻生物量之间存在良好的相关性。第三个池塘的相关性较差是由于小型浮游蓝藻频繁占优势。CPC 与细胞计数相关性较差，Chl-a 也不能很好地衡量蓝藻的存在。荧光法测量的 CPC 与任何时候池塘中占优势的蓝藻分类群有关。荧光法在蓝藻生物量的环境监测中有很好的应用潜力，但可能需要基于适用于当地水体的预定关系。基于 CPC 浓度的管理指南也将增强原位 CPC 测量的有用性。
Zotero 插件