温度与抑肽酶对维持促肾上腺皮质激素分析前稳定性的有效性比较
The Effectiveness of Temperature Versus Aprotinin in Maintaining the Preanalytical Stability of Adrenocorticotrophin.
作者信息
Turkon Hakan, Toprak Burak, Yalcin Hulya, Colak Ayfer, Ozturk Nurinnisa
机构信息
Department of Medical Biochemistry, Canakkale Onsekiz Mart University, Faculty of Medicine, Canakkale, Turkey.
Department of Clinical Biochemistry, Silopi State Hospital, Sırnak, Turkey
出版信息
Lab Med. 2016 Nov;47(4):279-282. doi: 10.1093/labmed/lmw027. Epub 2016 Sep 4.
OBJECTIVE
To investigate the effect of temperature and protease inhibitor on adrenocorticotrophin (ACTH) stability.
METHODS
We collected blood specimens from 10 donors into K3 EDTA (ethylenediaminetetraacetic acid) and K3 EDTA + aprotinin tubes. Plasma specimens were held at room temperature and at 2 °C to 8[Formula: see text] for 0, 2, 4, 8, 24, and 72 hours. ACTH concentrations in 4 storage conditions were compared with repeated-measures analysis of variance (ANOVA) at each time point.
RESULTS
ACTH concentrations substantially decreased after a 72-hour time period. At the 72nd hour, standard EDTA tubes and EDTA + aprotinin tubes stored at 4[Formula: see text] showed the optimal stability. EDTA + aprotinin tubes stored at room temperature performed more strongly than standard EDTA tubes stored at room temperature.
CONCLUSION
Our results show that refrigerated storage is more effective than protease inhibitor addition to maintain ACTH stability. We recommend using aprotinin tubes, especially for specimens standing at room temperature.
目的
研究温度和蛋白酶抑制剂对促肾上腺皮质激素（ACTH）稳定性的影响。
方法
我们从10名捐赠者采集血液标本，分别置于含K3乙二胺四乙酸（EDTA）管和含K3 EDTA +抑肽酶管中。血浆标本分别在室温以及2℃至8℃下保存0、2、4、8、24和72小时。在每个时间点，采用重复测量方差分析（ANOVA）比较4种保存条件下的ACTH浓度。
结果
72小时后ACTH浓度大幅下降。在第72小时，4℃保存的标准EDTA管和EDTA +抑肽酶管显示出最佳稳定性。室温下保存的EDTA +抑肽酶管比室温下保存的标准EDTA管表现更好。
结论
我们的结果表明，冷藏保存比添加蛋白酶抑制剂更能有效维持ACTH稳定性。我们建议使用抑肽酶管，尤其是对于室温下放置的标本。
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