College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China; Tianjin Engineering Center of Environmental Diagnosis and Contamination Remediation, Tianjin 300071, China; Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin 300071, China.
J Environ Sci (China). 2016 Sep;47:7-13. doi: 10.1016/j.jes.2015.12.023. Epub 2016 Mar 4.
The present work aimed to develop a novel strategy to bioremediate the petroleum hydrocarbon contaminants in the environment. Salt tolerant bacterium was isolated from Dagang oilfield, China and identified as Corynebacterium variabile HRJ4 based on 16S rRNA gene sequence analysis. The bacterium had a high salt tolerant capability and biochar was developed as carrier for the bacterium. The bacteria with biochar were most effective in degradation of n-alkanes (C16, C18, C19, C26, C28) and polycyclic aromatic hydrocarbons (NAP, PYR) mixture. The result demonstrated that immobilization of C. variabile HRJ4 with biochar showed higher degradation of total petroleum hydrocarbons (THPs) up to 78.9% after 7-day of incubation as compared to the free leaving bacteria. The approach of this study will be helpful in clean-up of petroleum-contamination in the environments through bioremediation process using eco-friendly and cost effective materials like biochar.
本工作旨在开发一种新策略，以生物修复环境中的石油烃污染物。从中国大港油田分离到一株耐盐菌，并根据 16S rRNA 基因序列分析鉴定为可变棒状杆菌 HRJ4。该菌具有较高的耐盐能力，并将生物炭开发为细菌的载体。细菌与生物炭的混合物在降解正构烷烃（C16、C18、C19、C26、C28）和多环芳烃（NAP、PYR）混合物方面最为有效。结果表明，与游离菌相比，固定化 HRJ4 后，在 7 天的孵育期内，总石油烃（THP）的降解率高达 78.9%。本研究的方法将有助于通过使用生物炭等环保且经济有效的材料进行生物修复过程，来清除环境中的石油污染。
