类肽-蛋白质偶联物的免疫原性。

The Immunogenicity of Peptoid-Protein Conjugates.

作者信息

Case Allison, Desmond Angela, Lopes Daniel, Dye Kelly, Mapes Kelly, Ruback Stephen, Pop Iliodora, Kim Jiyeun Kate, Chakravarty Pavitra, Smallshaw Joan E, Pop Laurentiu M, Vitetta Ellen S

机构信息

Cancer Immunobiology Center and Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Department of Bioengineering, The University of Texas at Dallas, Texas, USA.

Cancer Immunobiology Center and Department of Immunology, University of Texas Southwestern Medical Center, Dallas, Texas, USA; Department of Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.

出版信息

J Vaccines Vaccin. 2016 Aug;7(4). doi: 10.4172/2157-7560.1000329. Epub 2016 Jul 11.

DOI:10.4172/2157-7560.1000329

PMID:27595050

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5004597/

Abstract

We demonstrate that a peptoid composed of five monomers and attached a maleimide linker to a carrier protein elicits anti-peptoid, anti-linker and anti-carrier antibodies in rabbits. Specific anti-peptoid antibodies were affinity purified and used to reproducibly retrieve three specific peptoid-coupled beads from 20,000 irrelevant peptoid-beads using magnetic screening.

摘要

我们证明，由五个单体组成并连接有马来酰亚胺接头至载体蛋白的拟肽在兔体内引发了抗拟肽、抗接头和抗载体抗体。特异性抗拟肽抗体经亲和纯化后，通过磁性筛选从20,000个无关拟肽珠中可重复地检索出三种特异性拟肽偶联珠。