Ralfkiaer E, Pulford K A, Lauritzen A F, Avnstrøm S, Guldhammer B, Mason D Y
Department of Pathology, Rigshospitalet, University of Copenhagen, Denmark.
Histopathology. 1989 Jun;14(6):637-43. doi: 10.1111/j.1365-2559.1989.tb02205.x.
The reactivity of a monoclonal anti-neutrophil elastase antibody (NP57) with routinely processed biopsy samples from various acute leukaemias has been examined and compared with that of chloroacetate esterase and CD15 (hapten X), two other myeloid cell-associated markers detectable in paraffin sections. No staining was seen with these markers in 14 cases of acute lymphoblastic leukaemia. In contrast the neoplastic cells in 27 of 37 acute myeloid leukaemias were NP57 positive. Twenty of these were also positive for chloroacetate esterase, whereas CD15 was expressed in only six cases. These results indicate that detection of elastase with monoclonal NP57 forms a useful supplement to traditional methods for the histopathological diagnosis of acute myeloid leukaemias.
研究了一种单克隆抗中性粒细胞弹性蛋白酶抗体（NP57）与各种急性白血病常规处理活检样本的反应性，并将其与氯乙酸酯酶和CD15（半抗原X）这两种在石蜡切片中可检测到的其他髓系细胞相关标志物的反应性进行了比较。14例急性淋巴细胞白血病患者的这些标志物未见染色。相比之下，37例急性髓系白血病中有27例的肿瘤细胞NP57呈阳性。其中20例氯乙酸酯酶也呈阳性，而CD15仅在6例中表达。这些结果表明，用单克隆NP57检测弹性蛋白酶对急性髓系白血病的组织病理学诊断形成了传统方法的有益补充。
