Kramps J A, van der Valk P, van der Sandt M M, Lindeman J, Meijer C J
J Histochem Cytochem. 1984 Apr;32(4):389-94. doi: 10.1177/32.4.6561228.
The immunohistochemical results obtained with antibodies directed against human neutrophil elastase is described. It was found that neutrophil elastase can be used as a specific marker of cells of the neutrophilic lineage. In normal hematopoietic cell preparations, only promyelocytes and more differentiated myeloid cells stain positive for elastase. In acute or chronic myeloid and myelomonocytic leukemia, the same neutrophil myeloid cells stain positive, whereas neoplastic cells of the monocytoid line are negative. Using elastase in conjunction with other markers, it is possible to differentiate easily the involvement of different cell lines in myeloproliferative diseases.
描述了用针对人中性粒细胞弹性蛋白酶的抗体所获得的免疫组织化学结果。发现中性粒细胞弹性蛋白酶可作为嗜中性粒细胞系细胞的特异性标志物。在正常造血细胞制剂中，只有早幼粒细胞和分化程度更高的髓系细胞对弹性蛋白酶染色呈阳性。在急性或慢性髓系白血病和粒单核细胞白血病中，同样的中性粒细胞髓系细胞染色呈阳性，而单核细胞系的肿瘤细胞呈阴性。将弹性蛋白酶与其他标志物结合使用，可以很容易地区分不同细胞系在骨髓增殖性疾病中的受累情况。
