Pulford K A, Erber W N, Crick J A, Olsson I, Micklem K J, Gatter K C, Mason D Y
Nuffield Department of Pathology, John Radcliffe Hospital, Oxford.
J Clin Pathol. 1988 Aug;41(8):853-60. doi: 10.1136/jcp.41.8.853.
A monoclonal antibody, NP57, was produced and used against the neutrophil granule protein elastase, which selectively stain neutrophils in cryostat and paraffin wax sections. The antibody stains neutrophils and a subpopulation of monocytes in blood smears and neutrophil precursors in bone marrow smears, and gives positive reactions with the cell lines HL60 and U-937. It labelled the blast cells in 68% of cases of acute myeloid leukaemia (M1-M5) but was unreactive with all cases of lymphoid leukaemias. Most of the elastase negative myeloid leukaemias were labelled by monoclonal anti-myeloperoxidase (antibody MPO-7) as were cells from the promyelocytic line HL60. No cases of myeloid leukaemia showed the opposite pattern--that is elastase positive, myeloperoxidase negative, suggesting that the production of myeloperoxidase precedes the onset of elastase synthesis during myeloid maturation. The anti-elastase antibody NP57 is a useful addition to the range of monoclonal antibodies available for the differential diagnosis of acute leukaemia by alkaline phosphatase-antialkaline phosphatase (APAAP) labelling of cell smears; it may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections.
制备了一种单克隆抗体NP57，用于对抗中性粒细胞颗粒蛋白弹性蛋白酶，该抗体可在低温恒温器切片和石蜡切片中选择性地对中性粒细胞进行染色。该抗体可对血液涂片中的中性粒细胞和单核细胞亚群以及骨髓涂片中的中性粒细胞前体进行染色，并与HL60和U - 937细胞系产生阳性反应。它在68%的急性髓系白血病（M1 - M5）病例中标记原始细胞，但对所有淋巴白血病病例均无反应。大多数弹性蛋白酶阴性的髓系白血病被抗髓过氧化物酶单克隆抗体（抗体MPO - 7）标记，早幼粒细胞系HL60的细胞也是如此。没有髓系白血病病例呈现相反的模式，即弹性蛋白酶阳性、髓过氧化物酶阴性，这表明在髓系成熟过程中髓过氧化物酶的产生先于弹性蛋白酶合成的开始。抗弹性蛋白酶抗体NP57是通过细胞涂片的碱性磷酸酶 - 抗碱性磷酸酶（APAAP）标记对急性白血病进行鉴别诊断的可用单克隆抗体范围中的一个有用补充；它对于髓系白血病肿瘤沉积物的组织病理学诊断以及石蜡切片中中性粒细胞的检测也可能有价值。
