[改性正畸粘合剂的蛋白质排斥和抗菌性能]
[Protein-repellent and antibacterial properties of modified orthodontic adhesive].
作者信息
Zhang N, Ma Y S, Xu H K, Bai Y X
机构信息
Department of Orthodontics, Capital Medical University School of Stomatology, Beijing 100050, China.
Biomaterials & Tissue Engineering Division, University of Maryland Dental School, Baltimore, MD 21201, U S A.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2016 Sep;51(9):558-61. doi: 10.3760/cma.j.issn.1002-0098.2016.09.010.
OBJECTIVE
To develop a novel protein-repellent orthodontic adhesive by incorporating 2-methacryloyloxyethyl phosphorylcholine(MPC).
METHODS
MPC was incorporated into a commercially available orthodontic adhesive(Fuji ORTHO) at 0% (control), 1.5%, 3.0%, and 5.0% by mass. Enamel shear bond strength(SBS) was determined. Protein adsorption onto specimens was determined by a micro bicinchoninic acid method. A dental plaque microcosm biofilm model with human saliva as inoculum was used to investigate biofilm viability.
RESULTS
The SBS was not reduced in the group(3.0% MPC), compared to the control group. The amount of protein adsorption in the group(3.0% MPC) was (0.46±0.06) μg/cm(2) and (4.57 ± 0.42) μg/cm(2) in the control group. Lactic acid production of biofilms in the group(3.0% MPC) was (7.12±1.03) mmol/L and (12.16±1.24) mmol/L in the control group.
CONCLUSIONS
MPC based orthodontic adhesive greatly reduced the protein adsorption and bacterial adhesion, without compromising enamel shear bond strength.
目的
通过加入2-甲基丙烯酰氧乙基磷酰胆碱（MPC）来开发一种新型的具有蛋白质排斥性的正畸粘合剂。
方法
将MPC按质量分数0%（对照组）、1.5%、3.0%和5.0%加入到一种市售正畸粘合剂（Fuji ORTHO）中。测定釉质剪切粘结强度（SBS）。采用微量双辛可宁酸法测定标本上的蛋白质吸附情况。以人唾液为接种物，利用牙菌斑微生态生物膜模型研究生物膜活力。
结果
与对照组相比，3.0%MPC组的SBS未降低。3.0%MPC组的蛋白质吸附量为（0.46±0.06）μg/cm²，对照组为（4.57±0.42）μg/cm²。3.0%MPC组生物膜的乳酸产量为（7.12±1.03）mmol/L，对照组为（12.16±1.24）mmol/L。
结论
基于MPC的正畸粘合剂能显著降低蛋白质吸附和细菌粘附，且不影响釉质剪切粘结强度。
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