[2-甲基丙烯酰氧乙基磷酰胆碱对牙科粘合剂蛋白质排斥性能的影响]
[Effect of 2-methacryloyloxyethyl phosphorylcholine on the protein-repellent property of dental adhesive].
作者信息
Zhang Ning, Zhang Ke, Xu Huakun, Bai Yuxing
机构信息
Department of Orthodontics, Capital Medical University School of Stomatology, Beijing 100050, China.
Biomaterials & Tissue Engineering Division, University of Maryland Dental School, Baltimore, MD 21201, U S A.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2016 Mar;51(3):172-5. doi: 10.3760/cma.j.issn.1002-0098.2016.03.009.
OBJECTIVE
To evaluate the effect of 2-methacryloyloxyethyl phosphorylcholine (MPC) and nanoparticles of amorphous calcium phosphate (NACP) on the protein-repellent property of dental adhesive.
METHODS
MPC and NACP were incorporated into SBMP as the test group. Scotchbond Multi-Purpose (SBMP) was used as control group. Human dentin shear bond strengths were measured. Protein adsorption onto samples was determined by micro bicinchoninic acid (BCA) method. A dental plaque microcosm biofilm model with human saliva as inoculum was used to investigate biofilm viability.
RESULTS
The dentin bond strength of modified group was (28.7±2.2) MPa, which was not significantly different from that of the SBMP control group. The amount of protein adsorption in the modified group and the SBMP control group were (0.21±0.02) µg/cm(2) and (4.17±0.45) µg/cm(2) respectively. Lactic acid production of biofilms in modified group and SBMP control were (7.71 ± 1.01) mmol/L and (19.18 ± 2.34) mmol/L repectively.
CONCLUSIONS
MPC-NACP based dental adhesive greatly reduce the protein adsorption and bacterial adhesion, without compromising dentin shear bond strength. This novel bonding agent may have wide application.
目的
评估2-甲基丙烯酰氧乙基磷酰胆碱（MPC）和无定形磷酸钙纳米颗粒（NACP）对牙科粘合剂蛋白质排斥性能的影响。
方法
将MPC和NACP加入到Scotchbond多功能粘合剂（SBMP）中作为试验组。以Scotchbond多功能粘合剂（SBMP）作为对照组。测量人牙本质剪切粘结强度。采用微量双辛可宁酸（BCA）法测定样品上的蛋白质吸附量。使用以人唾液为接种物的牙菌斑微观生物膜模型来研究生物膜活力。
结果
改性组的牙本质粘结强度为（28.7±2.2）MPa，与SBMP对照组无显著差异。改性组和SBMP对照组的蛋白质吸附量分别为（0.21±0.02）μg/cm²和（4.17±0.45）μg/cm²。改性组和SBMP对照组生物膜的乳酸产量分别为（7.71±1.01）mmol/L和（19.18±2.34）mmol/L。
结论
基于MPC-NACP的牙科粘合剂可大大减少蛋白质吸附和细菌粘附，而不影响牙本质剪切粘结强度。这种新型粘合剂可能有广泛的应用。
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