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用于区分阿拉比卡和罗布斯塔烘焙咖啡粉的低场（1）H核磁共振光谱法。

Low-field (1)H NMR spectroscopy for distinguishing between arabica and robusta ground roast coffees.

作者信息

Defernez Marianne, Wren Ella, Watson Andrew D, Gunning Yvonne, Colquhoun Ian J, Le Gall Gwénaëlle, Williamson David, Kemsley E Kate

机构信息

Analytical Sciences Unit, Institute of Food Research, Norwich Research Park, Norwich NR4 7UA, UK.

Oxford Instruments, Tubney Woods, Abingdon, Oxford OX13 5QX, UK.

出版信息

Food Chem. 2017 Feb 1;216:106-13. doi: 10.1016/j.foodchem.2016.08.028. Epub 2016 Aug 11.

DOI:10.1016/j.foodchem.2016.08.028
PMID:27596398
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5055110/
Abstract

This work reports a new screening protocol for addressing issues of coffee authenticity using low-field (60MHz) bench-top (1)H NMR spectroscopy. Using a simple chloroform-based extraction, useful spectra were obtained from the lipophilic fraction of ground roast coffees. It was found that 16-O-methylcafestol (16-OMC, a recognized marker compound for robusta beans) gives rise to an isolated peak in the 60MHz spectrum, which can be used as an indicator of the presence of robusta beans in the sample. A total of 81 extracts from authenticated coffees and mixtures were analysed, from which the detection limit of robusta in arabica was estimated to be between 10% and 20% w/w. Using the established protocol, a surveillance exercise was conducted of 27 retail samples of ground roast coffees which were labelled as "100% arabica". None were found to contain undeclared robusta content above the estimated detection limit.

摘要

本研究报告了一种新的筛查方案，该方案利用低场（60MHz）台式¹H核磁共振波谱法解决咖啡真伪问题。通过基于氯仿的简单萃取，从研磨烘焙咖啡的亲脂性部分获得了有用的光谱。研究发现，16-O-甲基咖啡醇（16-OMC，一种公认的罗布斯塔咖啡豆标记化合物）在60MHz光谱中产生一个孤立峰，可作为样品中罗布斯塔咖啡豆存在的指标。共分析了81份来自经认证咖啡和混合物的提取物，据此估计阿拉比卡咖啡中罗布斯塔咖啡豆的检测限在10%至20%（w/w）之间。使用既定方案，对27份标记为“100%阿拉比卡”的研磨烘焙咖啡零售样品进行了监测。结果发现，没有一个样品含有高于估计检测限的未申报罗布斯塔咖啡豆成分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/7b33b0748c8d/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/b624150c2b7f/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/0544f9e67a53/fx3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/b6d00e09bfd0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/dd059f3b09df/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/b768149ba763/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/987cb9034ce8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/7b33b0748c8d/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/b624150c2b7f/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/0544f9e67a53/fx3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/b6d00e09bfd0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/dd059f3b09df/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/b768149ba763/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/987cb9034ce8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50eb/5055110/7b33b0748c8d/gr5.jpg

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