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Eurofins Analytics France, 9 rue Pierre Adolphe Bobierre, B.P. 42301, F-44323 NANTES Cedex 3, France.
Food Chem. 2020 Nov 1;329:127129. doi: 10.1016/j.foodchem.2020.127129. Epub 2020 May 27.
The acknowledged marker of Robusta coffee, 16-O-methylcafestol (16-OMC), can be quantified by NMR as a mixture with 16-O-methylkahweol (16-OMK), which accounts for approximately 10% of the mixture. In the present study, we detected and quantified 16-O-methylated diterpenes (16-OMD) in 248 samples of green Coffea arabica beans by NMR. We did not observe any differences between genotypes introgressed by chromosomal fragments of Robusta and non-introgressed genotypes. Environmental effects suggesting a possible protective role of 16-OMD for adaptation, as well as genotypic effects that support a high heritability of this trait were observed. Altogether, our data confirmed the presence of 16-OMD in green Arabica at a level approximately 1.5% that of a typical Robusta, endorsing the validity of 16-OMD as a marker for the presence of Robusta.
罗布斯塔咖啡公认的标志物 16-O-甲基咖啡醇（16-OMC）可以通过 NMR 定量为与 16-O-甲基卡瓦醇（16-OMK）的混合物，其约占混合物的 10%。在本研究中，我们通过 NMR 检测并定量了 248 个绿色阿拉比卡咖啡豆样品中的 16-甲基二萜（16-OMD）。我们没有观察到由罗布斯塔染色体片段导入的基因型和未导入的基因型之间存在任何差异。观察到的环境影响表明 16-OMD 可能具有适应保护作用，以及支持该性状高度遗传力的基因型效应。总的来说，我们的数据证实了 16-OMD 在绿色阿拉比卡咖啡中的存在水平约为典型罗布斯塔的 1.5%，这支持了 16-OMD 作为罗布斯塔存在的标志物的有效性。
