放线菌酮和 Platencin 类似物的 Mutasynthetic 文库。
A Mutasynthetic Library of Platensimycin and Platencin Analogues.
机构信息
Department of Chemistry, ‡Department of Molecular Therapeutics, and §Natural Products Library Initiative at The Scripps Research Institute, The Scripps Research Institute , Jupiter, Florida 33458, United States.
出版信息
Org Lett. 2016 Sep 16;18(18):4606-9. doi: 10.1021/acs.orglett.6b02248. Epub 2016 Sep 6.
Abstract
Inactivation of ptmB1, ptmB2, ptmT2, or ptmC in Streptomyces platensis SB12029, a platensimycin (PTM) and platencin (PTN) overproducer, revealed that PTM and PTN biosynthesis features two distinct moieties that are individually constructed and convergently coupled to afford PTM and PTN. A focused library of PTM and PTN analogues was generated by mutasynthesis in the ΔptmB1 mutant S. platensis SB12032. Of the 34 aryl variants tested, 18 were incorporated with high titers.
摘要
链霉菌 SB12029 是一种过度生产 platensimycin (PTM) 和 platencin (PTN) 的菌株，其 ptmB1、ptmB2、ptmT2 或 ptmC 的失活表明 PTM 和 PTN 生物合成具有两个独特的部分，它们分别构建并汇聚偶联以生成 PTM 和 PTN。通过在ΔptmB1 突变体 S. platensis SB12032 中的突变合成，生成了 PTM 和 PTN 类似物的聚焦文库。在所测试的 34 种芳基变体中，有 18 种变体以高浓度掺入。
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