Wang Denong
Tumor Glycomics Laboratory, SRI International Biosciences Division, Menlo Park, CA 94025, USA.
Microarrays (Basel). 2015 Nov 20;4(4):618-29. doi: 10.3390/microarrays4040618.
This report describes an experimental procedure for constructing integrated lipid, carbohydrate, and protein microarrays. In essence, it prints liposomes on nitrocellulose-coated micro-glass slides, a biochip substrate for spotting protein and carbohydrate microarrays, and the substances that can form liposomes (homo-liposomes) or can be incorporated into liposomes (hetero-liposomes) are suitable for microarray construction using existing microarray spotting devices. Importantly, this technology allows simultaneous detection of serum antibody activities among the three major classes of antigens, i.e., lipids, carbohydrates, and proteins. The potential of this technology is illustrated by its use in revealing a broad-spectrum of pre-existing anti-lipid antibodies in blood circulation and monitoring the epitope spreading of autoantibody reactivities among protein, carbohydrate, and lipid antigens in experimental autoimmune encephalomyelitis (EAE).
本报告描述了一种构建集成脂质、碳水化合物和蛋白质微阵列的实验方法。实质上，它是将脂质体打印在涂有硝酸纤维素的微玻片上，这是一种用于点样蛋白质和碳水化合物微阵列的生物芯片基质，能够形成脂质体的物质（同型脂质体）或可掺入脂质体的物质（异型脂质体）适用于使用现有的微阵列点样设备构建微阵列。重要的是，该技术能够同时检测血清中针对脂质、碳水化合物和蛋白质这三大类抗原的抗体活性。该技术的潜力通过其在揭示血液循环中广泛存在的抗脂质抗体以及监测实验性自身免疫性脑脊髓炎（EAE）中蛋白质、碳水化合物和脂质抗原之间自身抗体反应性的表位扩展方面的应用得到了体现。
