Yamaguchi Shoki, Aoyama Tomoki, Ito Akira, Nagai Momoko, Iijima Hirotaka, Tajino Junichi, Zhang Xiangkai, Wataru Kiyan, Kuroki Hiroshi
Department of Motor Function Analysis, Human Health Sciences, Graduate School of Medicine, Kyoto University, Kyoto, Japan; Japan Society for the Promotion of Science, Tokyo, Japan.
Department of Development and Rehabilitation of Motor Function, Human Health Sciences, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Ultrasound Med Biol. 2016 Dec;42(12):2903-2913. doi: 10.1016/j.ultrasmedbio.2016.07.021. Epub 2016 Sep 3.
We investigated the effect of low-intensity pulsed ultrasound (LIPUS) treatment combined with mesenchymal stromal cell (MSC) injection for cartilage repair and subchondral bone reconstitution for treatment of osteochondral defects. An osteochondral defect was created on both femur grooves of Wistar rats. Four weeks later, bone marrow MSCs were injected into the right knee joint. The rats were divided into two intervention groups: without or with LIPUS irradiation. Cartilage repair was evaluated histologically based on the Wakitani cartilage repair score. Subchondral bone reconstitution was evaluated as bone volume (BV)/tissue volume (TV) by micro-computed tomography analysis. MSC injection improved the cartilage repair score, and LIPUS irradiation improved BV/TV. Combination treatment promoted both cartilage repair and BV/TV improvement. Thus, MSC injection combined with LIPUS irradiation is more effective than either treatment alone in promoting concurrent cartilage repair and subchondral reconstitution.
我们研究了低强度脉冲超声（LIPUS）治疗联合间充质基质细胞（MSC）注射对软骨修复和软骨下骨重建的影响，以治疗骨软骨缺损。在Wistar大鼠的双侧股骨沟制造骨软骨缺损。四周后，将骨髓间充质干细胞注射到右膝关节。大鼠被分为两个干预组：不进行LIPUS照射或进行LIPUS照射。基于Wakitani软骨修复评分对软骨修复进行组织学评估。通过微型计算机断层扫描分析将软骨下骨重建评估为骨体积（BV）/组织体积（TV）。MSC注射提高了软骨修复评分，LIPUS照射提高了BV/TV。联合治疗促进了软骨修复和BV/TV的改善。因此，在促进同时进行软骨修复和软骨下重建方面，MSC注射联合LIPUS照射比单独任何一种治疗都更有效。
