Ziola B, Morhart M, Gilbert L, Karvonen B, Chen X P
Department of Microbiology, University of Saskatchewan, Saskatoon, Canada.
J Virol Methods. 1989 Jun;24(3):313-20. doi: 10.1016/0166-0934(89)90043-8.
Measles virus is usually grown in human or monkey fibroblast cells. We now show that LICR-LON-HMy2 (LL2) cells, a human plasma cell leukemia-derived line which grows in suspension culture, will permissively support replication of measles virus to an extent achievable with Vero cells. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of measles virions produced by LL2 cells showed a polypeptide pattern typical of measles virus. As well, measles virus-infected LL2 cells, like infected Vero cells, were found to secrete large amounts of virus hemagglutinin, but not other virus proteins. We thus conclude that LL2 cells can be effectively used to produce milligram amounts of measles virus and that virus-clarified culture medium from measles virus-infected LL2 cells is a potential source for purifying virus hemagglutinin.
麻疹病毒通常在人或猴成纤维细胞中培养。我们现在发现，LICR-LON-HMy2（LL2）细胞，一种源自人浆细胞白血病且能在悬浮培养中生长的细胞系，能够允许麻疹病毒在一定程度上进行复制，其复制程度与Vero细胞所能达到的相当。对LL2细胞产生的麻疹病毒颗粒进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，结果显示出典型的麻疹病毒多肽图谱。同样，与感染的Vero细胞一样，感染麻疹病毒的LL2细胞也被发现会分泌大量病毒血凝素，但不分泌其他病毒蛋白。因此，我们得出结论，LL2细胞可有效地用于生产毫克量的麻疹病毒，并且来自感染麻疹病毒的LL2细胞的病毒澄清培养基是纯化病毒血凝素的潜在来源。
