Cinatl J, Cinatl J, Rabenau H, Rapp J, Kornhuber B, Doerr H W
Centre of Hygiene, J. W. Goethe-University, Frankfurt a. M., Fed. Rep. of Germany.
Cell Biol Int. 1993 Sep;17(9):885-95. doi: 10.1006/cbir.1993.1152.
A protein-free chemically defined medium designated PFEK-1 was developed for culture of VERO cells on polyvinyl formal (PVF) culture surface without serum or other macromolecular supplements. VERO cells proliferated in PFEK-1 medium on PVF surface to a similar extent as cells in serum-supplemented medium without previous adaptation from serum-containing conditions. The protein-free culture infected with coxsackievirus B4, herpes simplex virus types 1 and 2, measles virus and poliovirus types 1, 2 and 3 developed viral titers comparable to those found in conventionally grown cells. The results demonstrated that VERO cells in protein-free culture provide a sensitive substrate for the production of human pathogenic viruses which are not contaminated by serum or other protein factors usually added to a culture medium.
一种名为PFEK-1的无蛋白化学限定培养基被开发出来，用于在聚乙烯醇缩甲醛（PVF）培养表面培养VERO细胞，无需血清或其他大分子补充剂。VERO细胞在PVF表面的PFEK-1培养基中增殖，其程度与在无血清补充培养基中的细胞相似，且无需事先从含血清条件下适应。用柯萨奇病毒B4、单纯疱疹病毒1型和2型、麻疹病毒以及脊髓灰质炎病毒1型、2型和3型感染的无蛋白培养物产生的病毒滴度与传统培养细胞中的相当。结果表明，无蛋白培养中的VERO细胞为生产人类致病病毒提供了一个敏感的底物，且不会被通常添加到培养基中的血清或其他蛋白质因子污染。
