Adam Eveline, Müller Henry, Erlacher Armin, Berg Gabriele
Institute of Environmental Biotechnology, Graz University of Technology, Petersgasse 12, 8010 Graz, Austria.
Stand Genomic Sci. 2016 Sep 6;11(1):61. doi: 10.1186/s40793-016-0185-3. eCollection 2016.
The Serratia plymuthica strains 3Rp8 and 3Re4-18 are motile, Gram-negative, non-sporulating bacteria. Strain 3Rp8 was isolated from the rhizosphere of Brassica napus L. and strain 3Re4-18 from the endorhiza of Solanum tuberosum L. Studies have shown in vitro activity against the soil-borne fungi Verticillium dahliae Kleb., Rhizoctonia solani Kühn, and Sclerotinia sclerotiorum. Here, we announce and describe the complete genome sequence of S. plymuthica 3Rp8 consisting of a single circular chromosome of 5.5 Mb that encodes 4954 protein-coding and 108 RNA-only encoding genes and of S. plymuthica 3Re4-18 consisting of a single circular chromosome of 5.4 Mb that encodes 4845 protein-coding and 109 RNA-only encoding genes. The whole genome sequences and annotations are available in NCBI under the locus numbers CP012096 and CP012097, respectively. The genome analyses revealed genes putatively responsible for the promising plant growth promoting and biocontrol properties including predicting factors such as secretion systems, iron scavenging siderophores, chitinases, secreted proteases, glucanases and non-ribosomal peptide synthetases, as well as unique genomic islands.
粘质沙雷氏菌菌株3Rp8和3Re4 - 18是运动性、革兰氏阴性、不产芽孢的细菌。3Rp8菌株从甘蓝型油菜根际分离得到，3Re4 - 18菌株从马铃薯内生根际分离得到。研究表明它们在体外对土传真菌大丽轮枝菌、立枯丝核菌和核盘菌具有活性。在此，我们公布并描述了粘质沙雷氏菌3Rp8的完整基因组序列，其由一条5.5 Mb的单一环状染色体组成，编码4954个蛋白质编码基因和108个仅编码RNA的基因；以及粘质沙雷氏菌3Re4 - 18的完整基因组序列，其由一条5.4 Mb的单一环状染色体组成，编码4845个蛋白质编码基因和109个仅编码RNA的基因。完整基因组序列和注释分别可在NCBI上通过登录号CP012096和CP012097获取。基因组分析揭示了可能负责其有前景的促进植物生长和生物防治特性的基因，包括预测因子如分泌系统、铁载体、几丁质酶、分泌型蛋白酶、葡聚糖酶和非核糖体肽合成酶，以及独特的基因组岛。
