Baker H, Grillo M, Margolis F L
Department of Neurology, Cornell University Medical College, White Plains, New York 10605.
J Comp Neurol. 1989 Jul 8;285(2):246-61. doi: 10.1002/cne.902850207.
Olfactory marker protein (OMP), previously thought to be expressed only by olfactory receptor neurons and their processes, was localized anatomically with immunocytochemical techniques to a number of brain regions in three rodent species, the mouse, rat, and hamster. In addition, the amount of antigen was quantified by radioimmunoassay (RIA) and characterized by an immunoblot procedure. In all three species the antigen could be detected immunocytochemically in the preoptic region and hypothalamus. The rat did not exhibit immunostaining in any other brain region. However, in the mouse neuronal labelling was observed throughout the neural axis, including cellular labelling in the bed nucleus of the anterior commissure, the median preoptic nucleus, the bed nucleus of the stria terminalis, the periventricular region, the anterior parvicellular subnucleus of the paraventricular nucleus, around the dorsomedial hypothalamic nucleus (pars compacta), the subincertal region, the arcuate nucleus, the anterior cortical nucleus of the amygdala, the suprageniculate nucleus, the lateral lemniscal nuclei, the lateraldorsal and lateralventral central gray, the posterior aspects of the commissural and marginal nuclei of the inferior colliculus, the paragenule nucleus, the A-5 region, the area postrema, the ventromedial nucleus of the solitary tract, area X, the spinal trigeminal nucleus (pars zonale), and superficial laminae of the spinal cord. The hamster displayed a different pattern of labelling including cells in the periventricular gray, the pontine reticular tegmental nucleus, the A-5 region, the medial vestibular complex, the prepositus hypoglossal nucleus, the parvicellular reticular nucleus, the lateral paragigantocellular nucleus, the raphe obscuras, the lateral reticular nucleus, and the lateral nucleus of the cerebellum. Immunostaining was seen in fibers within the red nucleus and within mossy fibers of the cerebellum. OMP levels could only be quantified by radioimmunoassay in the olfactory bulb of the three species and in the hamster cerebellum where they were 1/1,000 of those determined in the olfactory bulb. The authenticity of OMP measured in the RIA and detected immunocytochemically was verified by a double-antibody immunoisolation/immunodetection procedure, which confirmed that the antigen being visualized had the molecular properties expected for OMP. In summary, these experiments demonstrate that authentic OMP exists in small groups of neurons in many areas of the central nervous system.
嗅觉标记蛋白（OMP），以前被认为仅由嗅觉受体神经元及其突起表达，通过免疫细胞化学技术在三种啮齿动物（小鼠、大鼠和仓鼠）的多个脑区进行了解剖定位。此外，通过放射免疫测定法（RIA）对抗原量进行了定量，并通过免疫印迹程序进行了表征。在所有这三个物种中，均可通过免疫细胞化学方法在视前区和下丘脑检测到抗原。大鼠在任何其他脑区均未表现出免疫染色。然而，在小鼠中，在整个神经轴上均观察到神经元标记，包括在前连合核、视前正中核、终纹床核、室周区、室旁核的前小细胞亚核、下丘脑背内侧核（致密部）周围、下丘核区、弓状核、杏仁核的前皮质核、上膝状体核、外侧丘系核、背外侧和腹外侧中央灰质、下丘连合核和边缘核的后部、旁颗粒核、A-5区、最后区、孤束核的腹内侧核、X区、三叉神经脊髓核（带状部）以及脊髓浅层。仓鼠表现出不同的标记模式，包括室周灰质、脑桥网状被盖核、A-5区、内侧前庭复合体、舌下前置核、小细胞网状核、外侧巨细胞旁核、中缝隐核、外侧网状核和小脑外侧核中的细胞。在红核内和小脑的苔藓纤维内可见免疫染色。仅通过放射免疫测定法可对这三个物种的嗅球以及仓鼠小脑中的OMP水平进行定量，其中仓鼠小脑中的OMP水平是嗅球中所测水平的1/1000。通过双抗体免疫分离/免疫检测程序验证了RIA中测定的以及免疫细胞化学检测到的OMP的真实性，该程序证实所观察到的抗原具有OMP预期的分子特性。总之，这些实验表明，在中枢神经系统的许多区域中，真正的OMP存在于小群神经元中。
