Yang Feng, Wang Xiao, Pan Bin-Bin, Su Xun-Cheng
State Key Laboratory of Elemento-Organic Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China.
Chem Commun (Camb). 2016 Oct 4;52(77):11535-8. doi: 10.1039/c6cc06114a. Epub 2016 Sep 7.
Single-armed DOTA-like phenylsulfonated pyridine derivatives are rigid and stable paramagnetic tags for site-specific labeling of proteins. Their reactions with a solvent-exposed protein thiol group generate a stable C-S bond and produce one single paramagnetic species in solution NMR. The generated large paramagnetic effects yield valuable long-range structural restraints for proteins.
单臂类DOTA苯基磺化吡啶衍生物是用于蛋白质位点特异性标记的刚性且稳定的顺磁标签。它们与溶剂暴露的蛋白质硫醇基团反应生成稳定的C-S键，并在溶液核磁共振中产生单一的顺磁物种。所产生的大的顺磁效应为蛋白质产生了有价值的远程结构限制。
