Gorlaeus Laboratories, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC, Leiden, The Netherlands.
Department of Chemistry, Fu Jen Catholic University, No. 510, Zhongzheng Rd., Xinzhuang Dist., New, Taipei City, 24205, Taiwan.
Angew Chem Int Ed Engl. 2019 Sep 9;58(37):13093-13100. doi: 10.1002/anie.201906049. Epub 2019 Aug 13.
Synthetic metal complexes can be used as paramagnetic probes for the study of proteins and protein complexes. Herein, two transition metal NMR probes (TraNPs) are reported. TraNPs are attached through two arms to a protein to generate a pseudocontact shift (PCS) using cobalt(II), or paramagnetic relaxation enhancement (PRE) with manganese(II). The PCS analysis of TraNPs attached to three different proteins shows that the size of the anisotropic component of the magnetic susceptibility depends on the probe surroundings at the surface of the protein, contrary to what is observed for lanthanoid-based probes. The observed PCS are relatively small, making cobalt-based probes suitable for localized studies, such as of an active site. The obtained PREs are stronger than those obtained with nitroxide spin labels and the possibility to generate both PCS and PRE offers advantages. The properties of TraNPs in comparison with other cobalt-based probes are discussed.
合成金属配合物可用作研究蛋白质和蛋白质复合物的顺磁探针。本文报道了两种过渡金属 NMR 探针（TraNPs）。TraNPs 通过两条臂连接到蛋白质上，使用钴（II）产生赝接触位移（PCS），或使用锰（II）产生顺磁弛豫增强（PRE）。连接到三种不同蛋白质的 TraNPs 的 PCS 分析表明，磁各向异性的各向异性分量的大小取决于蛋白质表面的探针环境，这与基于镧系元素探针的观察结果相反。观察到的 PCS 相对较小，使得基于钴的探针适合于局部研究，例如活性部位。获得的 PRE 比使用氮氧自由基自旋标记物获得的要强，并且能够产生 PCS 和 PRE 提供了优势。讨论了 TraNPs 与其他基于钴的探针的性质。
