Mihelić M, Kalbacher H, Hannappel E, Voelter W
Abteilung für Physikalische Biochemie, Universität Tübingen, F.R.G.
J Immunol Methods. 1989 Aug 15;122(1):7-13. doi: 10.1016/0022-1759(89)90328-1.
In order to obtain specific antibodies against thymosin beta 9 showing minimal cross-reactivity with the highly homologous peptide thymosin beta 4, the N-terminal fragment 1-14 of thymosin beta 9 was used for immunization. These antibodies have been tested in a competitive ELISA and show less than 1% cross-reactivity with thymosin beta 4. On the other hand, antibodies raised against the native thymosin beta 9 (1-14) cross-react 35% with thymosin beta 4. Specific antibodies against thymosin beta 9 are important for studying the concentration and localization of thymosin beta 9 in thymus and other bovine tissues because thymosin beta 9 is always accompanied by thymosin beta 4. Using N-terminal fragments of thymosin beta 4-like peptides may be a general approach for obtaining specific antibodies since this part of sequence is less conserved in thymosin beta 4-like peptides.
为了获得与高度同源的肽胸腺素β4交叉反应性最小的抗胸腺素β9特异性抗体，使用胸腺素β9的N端片段1-14进行免疫。这些抗体已在竞争性ELISA中进行测试，与胸腺素β4的交叉反应性小于1%。另一方面，针对天然胸腺素β9（1-14）产生的抗体与胸腺素β4的交叉反应性为35%。抗胸腺素β9特异性抗体对于研究胸腺素β9在胸腺和其他牛组织中的浓度和定位很重要，因为胸腺素β9总是伴随着胸腺素β4。使用胸腺素β4样肽的N端片段可能是获得特异性抗体的通用方法，因为该序列部分在胸腺素β4样肽中保守性较低。
